Description

n-Mal-n-Bis(Peg2-Nhs Ester) is a heterobifunctional, amine-reactive crosslinking reagent featuring a maleimide group and two NHS-activated PEG2 ester arms. This molecular architecture is engineered to facilitate the stable conjugation of biomolecules, such as antibodies or peptides, to other entities like surfaces, particles, or drugs, under mild aqueous conditions. It is an essential tool for researchers and developers in the fields of bioconjugation, diagnostics, and targeted drug delivery who require precise and efficient linker chemistry. The compound is supplied as a high-purity material suitable for sensitive life science applications.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via thiol-maleimide chemistry.
• Protein/Peptide Labeling: Modification of amine groups on proteins or peptides with fluorescent dyes, biotin, or other probes for detection and analysis.
• Surface Functionalization: Immobilization of biomolecules onto biosensor chips, microarray slides, or nanoparticle surfaces to create bioactive interfaces.
• PEGylation Reagent: Introduction of short, hydrophilic polyethylene glycol (PEG) spacers to improve solubility, stability, and pharmacokinetic properties of therapeutic molecules.
• Diagnostic Assay Development: Construction of immunoassay components, such as enzyme-antibody conjugates, for ELISA and other diagnostic platforms.
• Hydrogel Formation: Crosslinking of amine-containing polymers (e.g., chitosan, gelatin) to form biocompatible hydrogels for tissue engineering and drug release.

Basic Information

Product Name	n-Mal-n-Bis(Peg2-Nhs Ester)
CAS No.	2182601-73-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Bis-NHS-PEG2-maleimide; Maleimide-PEG2-bis-NHS ester; 1,11-Bis(2,5-dioxopyrrolidin-1-yl)-3,6,9-trioxa-12-azatetradec-13-en-14-oic acid maleimide; NHS-PEG2-Mal; PEG2 bis-NHS ester maleimide; Heterobifunctional PEG crosslinker, NHS-PEG2-Maleimide; Maleimide-PEG2-NHS ester dimer
EINECS	Contact for details

Quality Control

Our n-Mal-n-Bis(Peg2-Nhs Ester) is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in critical applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation. We provide a detailed Certificate of Analysis (COA) with every shipment, documenting key specifications such as assay purity, moisture content, and functional group activity. Our quality system is designed to support research, diagnostic, and pre-clinical development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.