Description

5-Bromo-4-Chloro-3-Indolyl 2-Acetamido-2-Deoxy-A-D-Galactopyranoside is a specialized chromogenic substrate used for the detection of glycosidase enzymes, particularly α-N-acetylgalactosaminidase. This compound matters because it provides a highly sensitive and specific colorimetric signal, enabling precise enzymatic analysis in research and diagnostic applications. It is primarily needed by biotechnology, pharmaceutical, and diagnostic kit manufacturers for applications in enzyme characterization, high-throughput screening, and histochemical staining.

Application

• Enzyme Activity Assays: As a chromogenic substrate for detecting and quantifying α-N-acetylgalactosaminidase activity in vitro.
• Diagnostic Development: A key component in the formulation of diagnostic kits for genetic disorders like Schindler disease, which involves deficiencies in this enzyme.
• Biochemical Research: Used in research laboratories to study enzyme kinetics, inhibition, and substrate specificity for glycoside hydrolases.
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify potential inhibitors or activators of target glycosidases.
• Histochemistry & Cell Biology: For the localization of enzyme activity in tissue sections or cell cultures, where cleavage yields an insoluble indigo dye.
• Microbiology: Used in microbial identification systems that rely on specific glycosidase profiles of bacteria.
• Quality Control: For the validation and potency testing of enzyme preparations in biomanufacturing.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl 2-Acetamido-2-Deoxy-A-D-Galactopyranoside
CAS No.	2149583-96-8
Molecular Formula	C16H18BrClN2O6
Molecular Weight	449.68 g/mol
Synonyms	X-α-GalNAc; 5-Bromo-4-chloro-3-indolyl α-N-acetylgalactosaminide; 5-Bromo-4-chloro-3-indolyl N-acetyl-α-D-galactosaminide; BCIP-α-GalNAc; α-N-Acetylgalactosaminidase substrate; Chromogenic α-GalNAc substrate; Indoxyl-α-GalNAc derivative; 2-Acetamido-2-deoxy-α-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl 2-Acetamido-2-Deoxy-A-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. We provide comprehensive analytical data, including HPLC purity, identity confirmation (IR, NMR), and tests for residual solvents and moisture. Certificates of Analysis (COA) are available for every batch upon request, supporting compliance with research and diagnostic development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.