Description

Propargyl-Peg4-Ch2Co2-Nhs is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a propargyl group for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry and an N-hydroxysuccinimide (NHS) ester for efficient amine conjugation. This compound is essential for creating stable, well-defined bioconjugates and functionalized materials with controlled spacing and solubility provided by the tetraethylene glycol spacer. It is primarily utilized by researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science for applications such as antibody-drug conjugate (ADC) synthesis, peptide modification, and surface functionalization.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of cytotoxic drugs, fluorophores, or other payloads to antibodies, proteins, or peptides via amine-NHS and click chemistry handles.
• Peptide and Protein Modification: Introduction of alkyne functional groups onto biomolecules for subsequent labeling, immobilization, or crosslinking.
• Surface Functionalization: Crafting biofunctional surfaces on chips, nanoparticles, or beads for biosensing, diagnostics, and cell culture applications.
• Polymer and Dendrimer Synthesis: Serving as a versatile building block for creating functionalized polymers, hydrogels, and dendritic structures with tailored properties.
• Chemical Biology Probes: Synthesis of activity-based probes or affinity tags for target identification and proteomics studies.
• Diagnostic Reagent Preparation: Conjugation of detection molecules (e.g., enzymes, dyes) to antibodies or other capture agents in assay development.

Basic Information

Item	Detail
Product Name	Propargyl-Peg4-Ch2Co2-Nhs
CAS No.	2144777-76-2
Molecular Formula	C₂₀H₃₁NO₁₀
Molecular Weight	445.46 g/mol
Synonyms	Propargyl-PEG4-acid-NHS ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-(prop-2-yn-1-yloxy)ethoxy]ethoxy]ethoxy]ethoxy]propanoate; Propargyl-O-(CH₂CH₂O)₄-CH₂CO₂-NHS; Propargyl-PEG4-NHS ester; Alkyne-PEG4-NHS ester; NHS-PEG4-Propargyl; N-(3-(2-(2-(2-(2-(Prop-2-yn-1-yloxy)ethoxy)ethoxy)ethoxy)ethoxy)propanoyloxy)succinimide; Propargyl tetraethylene glycol NHS ester
EINECS	Contact for details

Quality Control

Our Propargyl-Peg4-Ch2Co2-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance. Certificates of Analysis (COA) with detailed specifications are provided for every shipment. We support development under cGMP guidelines for pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize condensation and hydrolysis of the NHS ester.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.