Description

n-(Azido-Peg3)-n-Boc-Peg4-T-Butyl Ester CAS NO 2112731-94-7 is a heterobifunctional polyethylene glycol (PEG) linker featuring an azide group and a tert-butyl ester-protected carboxylic acid, separated by Boc-protected amine and PEG spacers. This compound is engineered for precision bioconjugation, enabling controlled, site-specific coupling of biomolecules and materials via click chemistry and subsequent deprotection strategies. It is an essential building block for researchers and manufacturers in pharmaceutical development, proteomics, and advanced materials science, facilitating the creation of targeted drug delivery systems, diagnostic probes, and functionalized surfaces.

Application

• Bioconjugation and ADC Development: Serves as a key linker for constructing antibody-drug conjugates (ADCs) and other biopharmaceuticals, connecting payloads to targeting moieties with controlled stoichiometry.
• Peptide and Protein Modification: Used to introduce PEG chains (PEGylation) onto peptides or proteins to enhance solubility, stability, and pharmacokinetic profiles.
• Click Chemistry Scaffolds: The azide terminus participates in copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) reactions for labeling, immobilization, and network formation.
• Polymer and Dendrimer Synthesis: Acts as a functional monomer or cross-linker in the synthesis of smart polymers, hydrogels, and dendritic structures for biomedical applications.
• Surface Functionalization: Enables the covalent attachment of bioactive molecules to nanoparticles, chips, and other material surfaces for biosensing and diagnostic platforms.
• Protecting Group Strategy: The Boc and t-butyl ester protecting groups allow for orthogonal deprotection, enabling sequential and selective synthetic steps in complex molecule assembly.

Basic Information

Product Name	n-(Azido-Peg3)-n-Boc-Peg4-T-Butyl Ester
CAS No.	2112731-94-7
Molecular Formula	C33H64N4O13
Molecular Weight	724.88 g/mol
Synonyms	1-Azido-3,6,9,12,15,18,21-heptaoxatetratriacontan-34-oic acid, 2-[(1,1-dimethylethoxy)carbonyl]amino]ethyl 1,1-dimethylethyl ester; 2,5,8,11,14,17,20-Heptaoxadocosan-22-oic acid, 23-azido-2-[(2-[[(1,1-dimethylethoxy)carbonyl]amino]ethoxy)ethyl] 1,1-dimethylethyl ester; Azido-PEG3-Boc-PEG4-t-butyl ester; Boc-NH-PEG4-Azide t-butyl ester; N-(Azido-PEG3)-N-Boc-PEG4-COOtBu; Heterobifunctional PEG linker, Azide/Boc-Amine/t-Butyl Ester; t-Butyl 23-azido-2,5,8,11,14,17,20-heptaoxa-3-[(2-[[(1,1-dimethylethoxy)carbonyl]amino]ethoxy)ethyl]docosanoate
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg3)-n-Boc-Peg4-T-Butyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry to ensure identity and consistency. We provide detailed Certificates of Analysis (COA) with every shipment, documenting key parameters such as assay, water content, and residual solvents. Our quality commitment supports applications in GMP-grade research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.