Description

n-(Azido-Peg3)-n-Boc-Peg3-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and an N-hydroxysuccinimide (NHS) ester, separated by a Boc-protected amine and PEG spacers. This compound is essential for creating stable, site-specific bioconjugates through click chemistry and amine coupling, enabling precise control over molecular architecture. It is primarily needed by researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced materials science for applications such as antibody-drug conjugate (ADC) synthesis and hydrogel formation.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of payloads (e.g., drugs, toxins, fluorophores) to antibodies, proteins, or peptides via sequential click chemistry and NHS-ester coupling.
• PEGylation Linker: Introduction of polyethylene glycol chains to biomolecules to enhance solubility, stability, and circulation half-life.
• Functional Material Synthesis: Building block for creating functionalized surfaces, smart hydrogels, and polymeric networks with controlled chemical functionality.
• Proteomics Research: Tool for labeling, crosslinking, or immobilizing proteins for analytical studies and affinity purification.
• Diagnostic Reagent Formulation: Key component in the development of diagnostic assays and imaging probes requiring stable, defined linkages.
• Click Chemistry Scaffold: The azide group enables efficient, bioorthogonal copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) reactions.

Basic Information

Product Name	n-(Azido-Peg3)-n-Boc-Peg3-Nhs Ester
CAS No.	2112731-51-6
Molecular Formula	C31H55N5O13
Molecular Weight	705.8 g/mol
Synonyms	Azido-PEG3-Boc-PEG3-NHS ester; 1-Azido-3,6,9,12,15,18,21-heptaoxatetratriacontan-34-oic acid 2,5-dioxopyrrolidin-1-yl ester, 24-[(tert-butoxycarbonyl)amino]-; Boc-NH-PEG3-azide NHS ester; N3-(Boc-N-amido-PEG3)-PEG3-NHS ester; Heterobifunctional PEG crosslinker, Azide-Boc-NH-PEG3-PEG3-NHS; Azido-PEG3-Boc-Amino-PEG3-NHS Ester; 2,5-Dioxopyrrolidin-1-yl 24-[(tert-butoxycarbonyl)amino]-1-azido-3,6,9,12,15,18,21-heptaoxatetratriacontanoate
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg3)-n-Boc-Peg3-Nhs Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional group integrity. We adhere to cGMP principles where applicable, and our quality systems are designed to meet the stringent requirements of pharmaceutical and biotech partners.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term storage, keep under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Integrity (NMR)	Azide & NHS ester present, Boc protection intact

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.