Description

n-(Biotin-Peg4)-n-Bis(Peg4-Acid) is a heterobifunctional polyethylene glycol (PEG) linker featuring a biotin group and two carboxylic acid termini. This compound is engineered to facilitate the controlled conjugation of biomolecules, enabling precise biotinylation and subsequent immobilization or detection via streptavidin-biotin interactions. It is a critical reagent for researchers and manufacturers in the fields of bioconjugation, diagnostics, and targeted drug delivery systems. The product is supplied with the CAS number 2112731-48-1.

Application

• Bioconjugation Reagent: For site-specific labeling of proteins, antibodies, peptides, and oligonucleotides with biotin tags.
• Diagnostic Assay Development: Used to create capture surfaces for ELISA, lateral flow assays, and biosensors by linking target molecules to streptavidin-coated plates or beads.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Serves as a model or functional component in the design of linkers for attaching cytotoxic payloads to targeting antibodies.
• Nanoparticle Functionalization: Enables the surface modification of liposomes, polymeric nanoparticles, or quantum dots with biotin for targeted delivery or imaging.
• Affinity Chromatography Ligand Synthesis: Used to synthesize ligands for immobilization on solid supports to create streptavidin-capturing columns.
• Crosslinking Agent: Acts as a water-soluble, flexible spacer to crosslink amine-containing molecules via its carboxylic acid groups.
• Proteomics and Biomolecular Research: A key tool for pull-down assays, protein complex isolation, and studying protein-protein interactions.

Basic Information

Product Name	n-(Biotin-Peg4)-n-Bis(Peg4-Acid)
CAS No.	2112731-48-1
Molecular Formula	C49H86N4O20S
Molecular Weight	1083.3 g/mol
Synonyms	Biotin-PEG4-Bis(PEG4-acid); Biotin-PEG4-tris-PEG4-acid; Biotin-PEG4-acid, PEG4-Biotin-PEG4-acid; 2,2',2'',2'''-(2-(2-(2-(2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)azanediyl)tetraacetic acid; Biotinylated PEG linker; Heterobifunctional PEG crosslinker; Biotin-PEG4-CH2CH2COOH (bis)
EINECS	Contact for details

Quality Control

Our n-(Biotin-Peg4)-n-Bis(Peg4-Acid) is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical characterization, including HPLC for purity assessment, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, water, and common organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.