Description

n-(m-Peg4)-N'-(Dbco-Peg4)-Cy5 is a bifunctional, heterobifunctional linker-dye conjugate designed for advanced bioconjugation and labeling applications. This compound integrates a cyanine 5 (Cy5) fluorophore with a meta-PEG4 amine and a DBCO-PEG4 moiety, enabling sequential, bioorthogonal click chemistry and amine-reactive coupling strategies. It is essential for researchers and developers in the life sciences sector who require precise, multi-step labeling of biomolecules such as antibodies, proteins, and oligonucleotides. The product is supplied under CAS number 2107273-76-5.

Application

• Bioconjugation & Probe Development: Creation of fluorescently labeled antibodies, proteins, peptides, and nucleic acids for detection and imaging.
• Bioorthogonal Labeling: Utilizes strain-promoted azide-alkyne cycloaddition (SPAAC) via the DBCO group for labeling azide-modified biomolecules in live cells or complex mixtures.
• Multimodal Imaging Agent Synthesis: Serves as a key building block for constructing agents used in fluorescence microscopy, in vivo imaging, and flow cytometry.
• Drug Delivery & ADC Research: Facilitates the development of antibody-drug conjugates (ADCs) and other targeted therapeutics by providing a cleavable, PEGylated linker with a tracking fluorophore.
• Diagnostic Assay Development: Used in the manufacture of reagents for ELISA, lateral flow assays, and other diagnostic platforms requiring high-sensitivity fluorescent detection.
• Materials Science & Surface Functionalization: Modification of nanoparticles, hydrogels, and biosensor surfaces with fluorescent properties for tracking and analysis.

Basic Information

Product Name	n-(m-Peg4)-N'-(Dbco-Peg4)-Cy5
CAS No.	2107273-76-5
Molecular Formula	C84H121N7O17S23+ • 3X- (Counterion dependent)
Molecular Weight	~1560 g/mol (Free base, approximate)
Synonyms	DBCO-PEG4-Cy5-m-PEG4-amine; Cy5-m-PEG4-DBCO-PEG4-amine; N-(m-PEG4)-N'-(DBCO-PEG4)-Cy5; Cyanine5-m-PEG4-DBCO-PEG4-NH2; 1-(14-{2-[2-(2-{2-[2-(2-{4-[(1Z,3Z,5E)-5-(1-{6-[2-(2-{2-[2-(2-{4-[(2,5-Dioxopyrrolidin-1-yl)oxy]-4-oxobutoxy}ethoxy)ethoxy]ethoxy}ethoxy)ethyl]-3,3-dimethyl-5-sulfo-1,3-dihydro-2H-indol-2-ylidene}ethylidene)-1-ethyl-4-(1-ethyl-3,3-dimethyl-5-sulfo-1,3-dihydro-2H-indol-2-ylidene)buta-1,3-dien-1-yl]phenoxy}ethoxy)ethoxy]ethoxy}ethoxy)ethyl]pyrrolidine-2,5-dione (DBCO variant); DBCO-Cy5-PEG4-amine linker; Heterobifunctional Cy5-PEG-DBCO linker.
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(Dbco-Peg4)-Cy5 is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, mass spectrometry (MS) confirmation, and functional group validation. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, identity, and other critical parameters to support your research and development compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the material under an inert atmosphere (e.g., argon or nitrogen) to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark blue to green solid or film
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Group Integrity	≥ 90% (by NMR or assay)
Solubility	Soluble in DMSO, DMF, water, alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.