Description

Bis-(N,N'-Peg4-Nhs Ester)-Cy5 is a high-purity, bifunctional cyanine dye derivative designed for advanced bioconjugation and labeling applications. This compound is engineered to provide a long, flexible PEG4 spacer arm, enabling efficient and stable crosslinking of biomolecules with minimal steric hindrance. It is an essential reagent for researchers and manufacturers in the fields of biopharmaceutical development, in vitro diagnostics (IVD), and advanced life science research, where precise and reliable fluorescent labeling is critical.

Application

• Antibody-Drug Conjugate (ADC) Development: Used as a fluorescent linker-payload for tracking and studying the pharmacokinetics and biodistribution of ADC candidates.
• Protein and Peptide Labeling: Enables the site-specific conjugation of the Cy5 fluorophore to proteins, antibodies, and peptides for fluorescence imaging, Western blotting, and flow cytometry.
• Nucleic Acid Probes: Facilitates the creation of highly sensitive fluorescent probes for FISH (Fluorescence In Situ Hybridization), real-time PCR, and microarray analysis.
• Nanoparticle Functionalization: Acts as a key component for labeling polymeric nanoparticles, liposomes, and quantum dots for targeted imaging and drug delivery studies.
• Biomaterial and Hydrogel Modification: Used to incorporate fluorescent markers into scaffolds and hydrogels for non-invasive tracking in tissue engineering applications.
• Diagnostic Assay Kits: Serves as a critical raw material in the manufacture of fluorescent immunoassay and lateral flow test kits.

Basic Information

Item	Detail
Product Name	Bis-(N,N'-Peg4-Nhs Ester)-Cy5
CAS No.	2107273-48-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5 Bis(NHS ester) PEG4; Bis-PEG4-Cy5-NHS ester; Cyanine5 Bis(PEG4 NHS Ester); Cy5 Bis-succinimidyl ester PEG4; Bis(NHS-PEG4)-Cy5; Cy5-PEG4-Bis-NHS; 1,1'-(PEG4)2-Cy5 Bis(NHS ester); Cy5 Bis-PEG4-NHS Crosslinker
EINECS	Contact for details

Quality Control

Our Bis-(N,N'-Peg4-Nhs Ester)-Cy5 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and production standards. Our quality commitment supports applications requiring GMP-grade materials for diagnostic and therapeutic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Extinction Coefficient	~250,000 M⁻¹cm⁻¹ (at λmax ~649 nm in methanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.