Description

n-(m-Peg4)-N'-(Azide-Peg4)-Cy3 is a heterobifunctional, fluorescent labeling reagent designed for bioorthogonal conjugation and biomolecule tracking. This compound integrates a cyanine 3 (Cy3) fluorophore with a methoxy-terminated tetraethylene glycol (m-PEG4) spacer and an azide-terminated tetraethylene glycol (Azide-PEG4) linker, enabling controlled, site-specific labeling via click chemistry. It is essential for researchers and developers in bioconjugation, proteomics, and advanced diagnostic assay development who require high-purity, water-soluble probes with defined linker lengths and minimal non-specific binding.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, proteins, and peptides via strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
• Fluorescent Probe Synthesis: Creation of targeted imaging agents for fluorescence microscopy (e.g., confocal, super-resolution) and in vitro diagnostics.
• Biomaterial and Hydrogel Functionalization: Incorporation of fluorescent tags into polymers, nanoparticles, and scaffolds for tracking and visualization in biomedical engineering.
• Nucleic Acid Labeling: Modification of oligonucleotides or other nucleic acid analogs for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Live-Cell Imaging: Labeling of cell surface proteins or glycans for dynamic tracking of cellular processes due to its water solubility and reduced cytotoxicity from PEG spacers.
• Drug Delivery System Analysis: Fluorescent tagging of drug carriers (e.g., liposomes, dendrimers) to study biodistribution, cellular uptake, and pharmacokinetics.

Basic Information

Product Name	n-(m-Peg4)-N'-(Azide-Peg4)-Cy3
CAS No.	2107273-38-9
Molecular Formula	C58H84N6O14
Molecular Weight	1089.3 g/mol (Contact for details)
Synonyms	Cy3-PEG4-Azide; mPEG4-Cy3-PEG4-N3; N-(Methoxy PEG4)-N'-(Azido PEG4)-Cy3; Azido-PEG4-Cy3-methoxy; Cyanine3 azide PEG4 methyl ether; 2,5,8,11-Tetraoxatridecan-13-amine, 13-[(1E,3E)-3-[1-[(2,5,8,11-tetraoxatridecan-13-yl)amino]-1-oxo-3-phenyl-2-propen-1-ylidene]-1-phenyl-2-propen-1-ylidene]-2,5,8,11-tetraoxatridecan-13-azide; Cy3 MeO-PEG4-PEG4-N3; Fluorescent PEG-azide crosslinker
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(Azide-Peg4)-Cy3 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical characterization to ensure identity, purity, and performance consistency for sensitive research applications. Certificates of Analysis (COA) detailing HPLC purity, spectral data (NMR, MS), and functional performance are provided to guarantee it meets the stringent requirements for bioconjugation and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere (argon or nitrogen) and allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~150,000 cm-1M-1 at λmax
Water Content (KF)	≤ 1.0%
Functional Performance (Labeling)	≥ 90% conjugation efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.