Description

N,N'-Bis-(Propargyl-Peg4)-Cy5 is a bifunctional, near-infrared fluorescent labeling reagent featuring terminal alkyne groups for bioorthogonal click chemistry conjugation. This compound is engineered to enable the precise tagging and visualization of biomolecules, offering deep tissue penetration and minimal background interference. It is essential for researchers and developers in bioconjugation, pharmaceutical R&D, and advanced diagnostic assay development who require high-performance, site-specific fluorescent probes.

Application

• Bioconjugation and Probe Development: Site-specific labeling of antibodies, proteins, peptides, and oligonucleotides via copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• In Vivo and In Vitro Imaging: A critical component for developing NIR fluorescent probes for deep-tissue imaging, cell tracking, and biodistribution studies.
• Drug Discovery & Development: Used to label drug candidates, linkers, or targeting moieties to study pharmacokinetics, receptor binding, and cellular uptake.
• Diagnostic Assays: Fabrication of high-sensitivity fluorescent lateral flow assays (LFAs), ELISA, and other immunoassays requiring stable, bright NIR signals.
• Materials Science: Functionalization of polymers, nanoparticles, and surfaces with fluorescent properties for sensing and biointerface engineering.
• Chemical Biology Research: Tool for metabolic labeling and activity-based protein profiling (ABPP) to study biological processes in real-time.

Basic Information

Item	Details
Product Name	N,N'-Bis-(Propargyl-Peg4)-Cy5
CAS No.	2107273-08-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy5 bis-Propargyl-PEG4; Bis-Propargyl-PEG4-Cy5; N,N'-Bis(propargyl-PEG4)-cyanine5; Cy5-PEG4-bis-alkyne; Cy5 alkyne PEG crosslinker; Bifunctional Cy5-PEG4-alkyne; Cy5 dye with dual PEG4-alkyne linkers; Cy5-PEG4-(propargyl)2
EINECS	Contact for details

Quality Control

Our N,N'-Bis-(Propargyl-Peg4)-Cy5 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.