Description

n-(m-Peg4)-N'-(Azide-Peg3)-Cy5 is a heterobifunctional, fluorescent labeling reagent designed for advanced bioconjugation and bioimaging applications. This compound integrates a near-infrared cyanine dye (Cy5) with a methoxy-terminated tetraethylene glycol (m-PEG4) spacer and an azide-functionalized triethylene glycol (Azide-PEG3) linker, enabling highly specific, bioorthogonal click chemistry. It is essential for researchers and developers in pharmaceutical R&D, diagnostic assay development, and advanced materials science who require precise, stable, and fluorescent tagging of biomolecules. The product is supplied under CAS No. 2107273-02-7.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and oligonucleotides via the reactive azide group for detection and quantification.
• Bioorthogonal Imaging Probes: Creation of targeted imaging agents for in vitro and in vivo near-infrared fluorescence (NIRF) imaging and microscopy.
• Click Chemistry Reagent: Used in copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions for site-specific bioconjugation.
• Drug Delivery System Development: Functionalization of nanoparticles, liposomes, and polymer-drug conjugates to track biodistribution and cellular uptake.
• Diagnostic Assay Development: Key component in developing fluorescent immunoassays (FLISA), flow cytometry reagents, and lateral flow tests.
• Live-Cell Imaging: Labeling of cell surface receptors or intracellular targets for real-time visualization and tracking in live cells.
• Biomaterial and Hydrogel Modification: Incorporation into polymeric scaffolds and hydrogels to create fluorescently traceable materials for tissue engineering.

Basic Information

Product Name	n-(m-Peg4)-N'-(Azide-Peg3)-Cy5
CAS No.	2107273-02-7
Molecular Formula	C58H84N6O13
Molecular Weight	1049.3 g/mol (Contact for exact value)
Synonyms	Cy5-m-PEG4-Azide-PEG3; mPEG4-Azide-PEG3-Cy5; Azide-PEG3-Cy5-mPEG4; N-(Methoxy PEG4)-N'-(Azido PEG3)-Cy5; Cyanine5 azide PEG derivative; Heterobifunctional PEG-Cy5-azide; 1-Methoxy-2,5,8,11-tetraoxatridecan-13-yl 3-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)propyl (2E)-2-((1E,3E)-5-((E)-2-((1E,3E)-3-(2-((E)-1-(5-carboxypentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(5-sulfonatopentyl)indol-1-ium-2-yl)vinyl)cyclohex-1-en-1-yl)ethylidene)-3,3-dimethyl-1-(5-sulfonatopentyl)indoline-2-carboxylate
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(Azide-Peg3)-Cy5 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and functional group analysis. We provide comprehensive Certificates of Analysis (COA) detailing purity, absorbance/fluorescence properties, and functional performance. Our quality commitment ensures batch-to-batch consistency for critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere (argon or nitrogen) and allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~250,000 cm⁻¹M⁻¹ at λmax (~649 nm)
Functional Group Integrity (Azide)	≥ 90% by NMR or IR
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, water, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.