Description

2,5-Dioxopyrrolidin-1-Yl 17-Oxo-4,7,10,13,19-Pentaoxa-16-Azadocos-21-Yn-1-Oate is a specialized heterobifunctional crosslinking reagent featuring an activated ester and an alkyne group, separated by a hydrophilic polyethylene glycol (PEG) spacer. This molecular architecture is critical for enabling efficient, site-specific bioconjugation under mild aqueous conditions, which is essential for maintaining biomolecule integrity. It is primarily utilized by researchers and manufacturers in biopharmaceutical development, proteomics, and advanced diagnostics for labeling proteins, peptides, and other biomolecules.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, enzymes, and other proteins with fluorescent dyes, biotin, or other probes via the activated NHS ester and alkyne groups.
• ADC (Antibody-Drug Conjugate) Linker Synthesis: Serves as a key building block for constructing cleavable or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Click Chemistry Reagent: The terminal alkyne enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) for highly efficient and selective coupling with azide-functionalized molecules.
• PEGylation Reagent: Used to introduce hydrophilic PEG chains onto drug molecules or surfaces to improve solubility, stability, and pharmacokinetic profiles.
• Surface Functionalization: Immobilization of biomolecules onto azide-presenting surfaces (e.g., chips, beads, nanoparticles) for biosensor and microarray development.
• Proteomics Research: Chemical proteomics applications for activity-based protein profiling (ABPP) and identifying enzyme-substrate interactions.
• Diagnostic Assay Development: Conjugation of detection molecules (e.g., enzymes, fluorophores) to antibodies or antigens in ELISA and lateral flow assays.
• Polymer and Material Science: Synthesis of functionalized polymers and hydrogels with tailored biological activity for biomedical applications.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 17-Oxo-4,7,10,13,19-Pentaoxa-16-Azadocos-21-Yn-1-Oate
CAS No.	2101206-92-0
Molecular Formula	C25H38N2O11
Molecular Weight	542.58 g/mol
Synonyms	NHS-PEG5-Alkyne; Alkyne-PEG5-NHS ester; 5k-PEG-Alkyne-NHS ester (Note: "5k" is a common misnomer for PEG5); N-Succinimidyl ester of PEG5-alkyne; 21-Azido-4,7,10,13,16-pentaoxa-19-azahenicosanoic acid 2,5-dioxo-1-pyrrolidinyl ester (alternative IUPAC); DBCO-PEG5-NHS ester (Note: This is a distinct but related compound); Heterobifunctional PEG linker, NHS-PEG-alkyne; Propargyl-PEG5-NHS
EINECS	Contact for details

Quality Control

Our 2,5-Dioxopyrrolidin-1-Yl 17-Oxo-4,7,10,13,19-Pentaoxa-16-Azadocos-21-Yn-1-Oate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, critical for sensitive bioconjugation workflows. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support compliance with cGMP and ISO 9001 standards for research and pharmaceutical development use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.