Description

4,7,10,16-Tetraoxa-13-Azanonadec-18-Ynoic Acid, 14-Oxo-, 2,5-Dioxo-1-Pyrrolidinyl Ester is a specialized heterobifunctional crosslinking agent featuring a terminal alkyne and an activated NHS ester. This compound is engineered for high-efficiency bioconjugation, enabling the stable and site-specific labeling of biomolecules under mild aqueous conditions. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and advanced materials science, facilitating the creation of novel diagnostics, targeted therapeutics, and functionalized surfaces.

Application

• Bioconjugation and Protein Labeling: Site-specific coupling of peptides, proteins, and antibodies via amine groups using the NHS ester moiety.
• Click Chemistry: Utilization of the terminal alkyne for copper-catalyzed azide-alkyne cycloaddition (CuAAC) to attach fluorescent dyes, PEG chains, or other functional molecules.
• ADC (Antibody-Drug Conjugate) Linker Synthesis: Serves as a key building block for constructing cleavable or non-cleavable linkers in targeted cancer therapies.
• Surface Functionalization: Modification of nanoparticles, biosensors, and microarray slides to create bioactive surfaces for assays.
• Polymer Chemistry: Incorporation into polymer backbones or as an end-group modifier to introduce reactive handles for further chemical elaboration.
• Probe and Diagnostic Reagent Development: Creation of molecular probes for imaging, detection, and diagnostic applications.

Basic Information

Product Name	4,7,10,16-Tetraoxa-13-Azanonadec-18-Ynoic Acid, 14-Oxo-, 2,5-Dioxo-1-Pyrrolidinyl Ester
CAS No.	2101206-78-2
Molecular Formula	C20H28N2O10
Molecular Weight	456.45 g/mol
Synonyms	NHS-PEG4-Alkyne; Alkyne-PEG4-NHS ester; 21-Azido-4,7,10,13,16-pentaoxahenicos-20-ynoic acid 2,5-dioxopyrrolidin-1-yl ester; Propargyl-PEG4-NHS; N-(3-Azidopropyl)-21-[(2,5-dioxo-1-pyrrolidinyl)oxy]-4,7,10,13,16-pentaoxa-20-azanonadec-20-yn-1-aminium; PEG4 Alkyne NHS Ester; Alkyne-PEG4-NHS; Heterobifunctional PEG crosslinker, NHS-PEG4-Alkyne
EINECS	Contact for details

Quality Control

Our 4,7,10,16-Tetraoxa-13-Azanonadec-18-Ynoic Acid, 14-Oxo-, 2,5-Dioxo-1-Pyrrolidinyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the NHS ester is susceptible to hydrolysis. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.