Description

3,6,9-Trioxa-12-Azapentadecanoic Acid, 15-(2,5-Dihydro-2,5-Dioxo-1H-Pyrrol-1-Yl)-13-Oxo-, 2,5-Dioxo-1-Pyrrolidinyl Ester is a heterobifunctional crosslinking agent featuring a hydrophilic tetraethylene glycol spacer, an amine-reactive NHS ester, and a maleimide group for thiol conjugation. This compound is engineered to enable the stable and site-specific conjugation of biomolecules, such as antibodies, peptides, and proteins, under mild aqueous conditions. It is an essential intermediate for researchers and manufacturers in bioconjugation, diagnostics, and advanced drug delivery system development.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key building block for constructing cleavable or non-cleavable linkers connecting cytotoxic payloads to monoclonal antibodies.
• PEGylation Reagent: Used to introduce polyethylene glycol (PEG) chains onto proteins or peptides to enhance solubility, stability, and circulation half-life.
• Diagnostic Probe Conjugation: Facilitates the labeling of proteins, antibodies, or oligonucleotides with fluorescent dyes, enzymes, or other reporter molecules for immunoassays and biosensors.
• Surface Functionalization: Enables the covalent attachment of biomolecules to nanoparticles, microspheres, biosensor chips, and other solid supports for assay development.
• Protein-Protein Crosslinking: Used in structural biology and biophysics studies to investigate protein interactions and complex formation.
• Peptide & Small Molecule Conjugation: Critical for creating targeted therapeutic agents by linking cell-penetrating peptides or targeting ligands to active pharmaceutical ingredients.
• Hydrogel Formation: Serves as a crosslinker in the synthesis of biocompatible hydrogels for tissue engineering and controlled release applications.
• Biomaterial Modification: Imparts specific biological activity to polymers and other biomaterials by grafting functional peptides or proteins.

Basic Information

Product Name	3,6,9-Trioxa-12-Azapentadecanoic Acid, 15-(2,5-Dihydro-2,5-Dioxo-1H-Pyrrol-1-Yl)-13-Oxo-, 2,5-Dioxo-1-Pyrrolidinyl Ester
CAS No.	2101206-45-3
Molecular Formula	C20H27N3O10
Molecular Weight	469.44 g/mol
Synonyms	Maleimide-PEG4-NHS ester; NHS-PEG4-Maleimide; SM(PEG)4; N-(2-{2-[2-(2-{3-[(2,5-Dioxopyrrolidin-1-yl)oxy]-3-oxopropoxy}ethoxy)ethoxy]ethoxy}ethyl)maleimide; 4-Arm PEG NHS Ester (Maleimide terminated); PEG-based heterobifunctional crosslinker, NHS-PEG4-MAL; N-(Methoxy PEG4)-N'-(succinimidyl propionate) maleimide
EINECS	Contact for details

Quality Control

Every batch of our 3,6,9-Trioxa-12-Azapentadecanoic Acid, 15-(2,5-Dihydro-2,5-Dioxo-1H-Pyrrol-1-Yl)-13-Oxo-, 2,5-Dioxo-1-Pyrrolidinyl Ester is manufactured and tested under a strict quality management system. We provide comprehensive analytical data including HPLC purity, NMR confirmation, and functional group assay to ensure product identity, purity, and performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with lot-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Due to its hygroscopic (moisture-sensitive) nature, store the product under an inert atmosphere (e.g., argon or nitrogen) in a freezer at -20°C or below. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (Maleimide)	≥ 90%
Functional Assay (NHS Ester)	≥ 90%
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.