Description

2,5-Dioxopyrrolidin-1-Yl 11-Oxo-4,7,13-Trioxa-10-Azahexadec-15-Yn-1-Oate is a specialized heterobifunctional crosslinking reagent featuring an activated ester and an alkyne group, designed for advanced bioconjugation and click chemistry applications. Its molecular architecture enables efficient and selective coupling of biomolecules, such as peptides, proteins, and oligonucleotides, under mild aqueous conditions. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and diagnostic assay production, where precise and stable molecular linkages are critical.

Application

• Bioconjugation Reagent: For coupling carboxylic acid-containing molecules (e.g., peptides, proteins) to alkyne-modified surfaces or biomolecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• ADC (Antibody-Drug Conjugate) Linker Synthesis: Serves as a key intermediate in constructing cleavable or non-cleavable linkers for targeted cancer therapies.
• Proteomic Probe Development: Used to create activity-based probes (ABPs) for labeling and studying enzyme function in complex biological samples.
• Polymer Functionalization: Enables the site-specific modification of polymers and dendrimers with bioactive ligands for drug delivery systems.
• Surface Immobilization: Facilitates the covalent attachment of biomolecules onto alkyne-presenting chips, beads, or slides for biosensor and microarray platforms.
• Diagnostic Reagent Manufacturing: Employed in the synthesis of stable, high-affinity conjugates for immunoassays (ELISA, lateral flow tests).
• Materials Science: Used to crosslink or functionalize hydrogels and other biomaterials to introduce specific biological cues.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 11-Oxo-4,7,13-Trioxa-10-Azahexadec-15-Yn-1-Oate
CAS No.	2101206-30-6
Molecular Formula	C19H26N2O9
Molecular Weight	426.42 g/mol
Synonyms	NHS-PEG4-Alkyne; N-Hydroxysuccinimide ester of PEG4 alkyne; 5-Isomer of NHS-PEG4-Alkyne; 17-((2,5-Dioxopyrrolidin-1-yl)oxy)-4,7,10,13-tetraoxo-3,6,9,12-tetraazaheptadec-16-ynoic acid; Alkyne-PEG4-NHS ester; 2,5-Dioxo-1-pyrrolidinyl 17-(1-oxo-2-propyn-1-yl)-4,7,10,13-tetraoxa-3,6,9,12-tetraazaheptadecanoate; PEG4 Alkyne NHS Ester; N-Succinimidyl-PEG4-alkyne
EINECS	Contact for details

Quality Control

Our 2,5-Dioxopyrrolidin-1-Yl 11-Oxo-4,7,13-Trioxa-10-Azahexadec-15-Yn-1-Oate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive research and development applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent hydrolysis of the active ester. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms (M+Na)+
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.