Description

n-(Azido-Peg2)-n-Boc-Peg4-Nhs Ester CAS NO 2093153-95-6 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and an N-hydroxysuccinimide (NHS) ester, separated by a Boc-protected amine spacer. This compound is engineered for efficient and selective bioconjugation, enabling the stable coupling of biomolecules via click chemistry and amine-reactive chemistry in a controlled, sequential manner. It is an essential reagent for researchers and developers in biopharmaceuticals, proteomics, and advanced material science who require precise and stable linkages for drug delivery systems, diagnostic assays, and surface functionalization.

Application

• ADC (Antibody-Drug Conjugate) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via click chemistry.
• PEGylation of Peptides and Proteins: To introduce PEG chains for improving pharmacokinetic properties such as solubility, stability, and circulation half-life.
• Surface Functionalization: For modifying nanoparticles, biosensors, and microarray slides with bioactive ligands.
• Polymer Chemistry: As a building block for synthesizing functionalized, crosslinked hydrogels and smart polymers.
• Diagnostic Reagent Synthesis: In the creation of fluorescent or enzyme-labeled probes for immunoassays and imaging.
• Biomaterial Engineering: To create bioactive scaffolds for tissue engineering with controlled cell-adhesion properties.
• Protecting Group Strategy: The Boc-protected amine allows for orthogonal deprotection and further functionalization in multi-step synthetic schemes.

Basic Information

Product Name	n-(Azido-Peg2)-n-Boc-Peg4-Nhs Ester
CAS No.	2093153-95-6
Molecular Formula	C31H56N6O13
Molecular Weight	720.82 g/mol
Synonyms	Azido-PEG2-Boc-PEG4-NHS ester; Boc-N-amido-PEG4-NHS ester-PEG2-azide; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]-3-[(2-methylpropan-2-yl)oxycarbonylamino]propoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; N-(Azido-PEG2)-N-Boc-PEG4-NHS Ester; Heterobifunctional PEG Crosslinker, Azide-Boc-NHS; Boc-NH-PEG4-NHS-PEG2-Azide; 1-Boc-3-azido-7,10,13,16,19,22,25,28-octaoxa-4-azahentriacontan-31-oic acid N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg2)-n-Boc-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and functional integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize condensation and hydrolysis of the active NHS ester.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (NHS Ester)	≥ 90% of theoretical
Functional Group Assay (Azide)	≥ 90% of theoretical

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.