Description

n-Boc-n-Bis(Peg4-Nhs Ester) CAS NO 2093153-08-1 is a high-purity, heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a Boc-protected amine. This compound is engineered for the precise and stable conjugation of biomolecules, enabling the creation of advanced drug delivery systems and diagnostic reagents. It is an essential building block for researchers and manufacturers in the pharmaceutical, biotech, and life sciences sectors developing next-generation therapeutics and bioconjugates.

Application

• Bioconjugation and Linker Chemistry: Used for the covalent attachment of peptides, proteins, antibodies, or other amine-containing molecules to surfaces, nanoparticles, or other polymers.
• ADC (Antibody-Drug Conjugate) Development: Serves as a key reagent for constructing stable, cleavable, or non-cleavable linkers between monoclonal antibodies and cytotoxic payloads.
• PEGylation Reagent: Facilitates the site-specific PEGylation of therapeutic proteins and peptides to enhance solubility, prolong circulation half-life, and reduce immunogenicity.
• Diagnostic Assay Development: Employed in the synthesis of enzyme conjugates, fluorescent probes, and other labeled biomolecules for ELISA, immunoassays, and biosensors.
• Hydrogel and Biomaterial Synthesis: Acts as a crosslinking agent in the fabrication of PEG-based hydrogels for tissue engineering, 3D cell culture, and controlled drug release matrices.
• Surface Functionalization: Used to modify nanoparticles, quantum dots, or solid supports with amine-reactive groups for subsequent biomolecule immobilization.

Basic Information

Item	Detail
Product Name	n-Boc-n-Bis(Peg4-Nhs Ester)
CAS No.	2093153-08-1
Molecular Formula	C33H58N2O16
Molecular Weight	738.82 g/mol
Synonyms	1,11-Bis-[(2,5-dioxo-1-pyrrolidinyl)oxy]-4,7,10,13,16,19,22,25,28,31-decaoxa-3,12-diazahentriacontane-3,12-dicarboxylic acid di-tert-butyl ester; Boc-NH-PEG4-NHS ester; tert-Butyl N-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[(2,5-dioxopyrrolidin-1-yl)oxy]-2-oxoethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl]carbamate; Boc-Amine-PEG4-NHS Ester; Heterobifunctional PEG Crosslinker, Boc-PEG4-NHS; NHS-PEG4-Boc; 1-tert-Butyl 12-(2,5-dioxopyrrolidin-1-yl) 3,6,9,12,15,18,21,24,27,30-decaoxadotriacontane-1,12-dioate
EINECS	Contact for details

Quality Control

Our n-Boc-n-Bis(Peg4-Nhs Ester) is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by NMR and Mass Spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, inert atmosphere. This product is hygroscopic (moisture-sensitive) and the NHS ester group is susceptible to hydrolysis. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.