Description

Propanoic Acid, 3-[2-[2-[2-[4-(6-Methyl-1,2,4,5-Tetrazin-3-Yl)Phenoxy]Ethoxy]Ethoxy]Ethoxy]-, 2,5-Dioxo-1-Pyrrolidinyl Ester is a specialized bifunctional chemical linker featuring a tetrazine moiety and an activated ester, designed for advanced bioconjugation and materials science applications. This compound matters for its ability to facilitate rapid, bioorthogonal click chemistry via inverse electron-demand Diels-Alder (IEDDA) reactions, enabling precise and efficient labeling of biomolecules and polymers. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and the creation of functionalized nanomaterials who require high-purity, reliable conjugation reagents.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Used as a key linker for site-specific conjugation of cytotoxic payloads to monoclonal antibodies via tetrazine-trans-cyclooctene (TCO) chemistry.
• Pre-Targeted Imaging and Therapy: Enables the construction of probes for in vivo pretargeting strategies in PET, SPECT, and fluorescence imaging, as well as radiopharmaceuticals.
• Protein and Peptide Labeling: Facilitates the covalent attachment of fluorophores, affinity tags, or other functional groups to proteins and peptides for analytical and diagnostic applications.
• Surface Functionalization and Material Science: Used to modify nanoparticles, polymers, and solid surfaces with tetrazine groups for subsequent click chemistry-based assembly or sensing applications.
• Chemical Biology and Proteomics Research: A critical tool for studying protein function, interactions, and localization through bioorthogonal labeling strategies in live cells.
• Polymer Chemistry: Serves as a functional monomer or cross-linker for synthesizing smart polymers and hydrogels with responsive or bioactive properties.

Basic Information

Product Name	Propanoic Acid, 3-[2-[2-[2-[4-(6-Methyl-1,2,4,5-Tetrazin-3-Yl)Phenoxy]Ethoxy]Ethoxy]Ethoxy]-, 2,5-Dioxo-1-Pyrrolidinyl Ester
CAS No.	2055646-22-3
Molecular Formula	C24H30N4O8
Molecular Weight	502.52 g/mol
Synonyms	6-Methyltetrazine-PEG4-NHS Ester; Tetrazine-PEG4-NHS Ester; 3-(6-Methyl-1,2,4,5-tetrazin-3-yl)-PEG4-NHS ester; MeTz-PEG4-NHS Ester; NHS-PEG4-Me-Tetrazine; TCO Click Chemistry Reagent; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenoxy]ethoxy]ethoxy]ethoxy]propanoate
EINECS	Contact for details

Quality Control

Every batch of our Propanoic Acid, 3-[2-[2-[2-[4-(6-Methyl-1,2,4,5-Tetrazin-3-Yl)Phenoxy]Ethoxy]Ethoxy]Ethoxy]-, 2,5-Dioxo-1-Pyrrolidinyl Ester is manufactured under strict quality management systems and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency. Our quality protocols are designed to meet the stringent requirements of research and pre-clinical development. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles via HPLC, NMR, and MS is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the primary container sealed in a secondary container with an appropriate desiccant. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to purple solid or powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.