Description

3-Azidopropionic Acid Sulfo-NHS Ester is a specialized heterobifunctional crosslinking reagent designed for bioconjugation applications. This compound matters for its ability to introduce azide groups into biomolecules, enabling subsequent bioorthogonal click chemistry reactions, such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is primarily needed by researchers and manufacturers in the fields of biopharmaceutical development, proteomics, and diagnostic assay production for labeling proteins, antibodies, and other biological entities.

Application

• Bioconjugation and Protein Labeling: Covalent attachment of azide handles to proteins, antibodies, and peptides for downstream modification.
• Click Chemistry Reagent: Key intermediate for CuAAC and strain-promoted azide-alkyne cycloaddition (SPAAC) reactions in chemical biology.
• Proteomics Research: Synthesis of activity-based probes (ABPs) and affinity tags for target identification and enrichment studies.
• ADC (Antibody-Drug Conjugate) Development: Facilitating the site-specific conjugation of cytotoxic payloads to monoclonal antibodies.
• Diagnostic Reagent Synthesis: Production of labeled antigens and antibodies for immunoassays (ELISA, lateral flow tests).
• Polymer and Material Science: Functionalization of surfaces, hydrogels, and nanoparticles with bio-reactive azide groups.
• Glycan Labeling: Modification of carbohydrates and glycoproteins for analytical and imaging purposes.

Basic Information

Product Name	3-Azidopropionic Acid Sulfo-NHS Ester
CAS No.	2055198-09-7
Molecular Formula	C9H11N5O7S
Molecular Weight	333.28 g/mol
Synonyms	3-Azidopropanoic Acid Sulfo-NHS Ester; N-Hydroxysulfosuccinimide Ester of 3-Azidopropionic Acid; Sulfo-NHS-Azide; Azido-PEG2-acid Sulfo-NHS Ester; 3-Azidopropionic Acid N-Hydroxysulfosuccinimide Ester; Azidoacetic Acid Sulfo-NHS Ester (common misnomer); 2-[2-[2-(2,5-Dioxopyrrolidin-1-yl)oxy]-2-oxoethoxy]ethoxy]ethyl azide (related structure); Sulfo-NHS Ester of β-Azidopropionic Acid
EINECS	Contact for details

Quality Control

Our 3-Azidopropionic Acid Sulfo-NHS Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency for sensitive research and development applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.