Description

Pc-Biotin-Peg4-Nhs Carbonate is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for researchers and manufacturers who require precise and stable attachment of biotinylated molecules to various substrates, such as proteins, peptides, or surfaces, via its amine-reactive NHS ester group. It is essential for professionals in pharmaceutical development, diagnostic assay manufacturing, and proteomics research who need reliable, high-quality linkers for creating sensitive detection systems and targeted therapeutic conjugates.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific biotinylation of antibodies or linker-payloads to facilitate purification, characterization, or creation of pre-targeting systems.
• Diagnostic Assay Fabrication: Immobilization of biotinylated capture antibodies or antigens onto streptavidin-coated microplates, beads, or biosensors for ELISA, lateral flow, and other immunoassays.
• Affinity Chromatography Resin Preparation: Functionalization of solid supports with biotin for capturing streptavidin/avidin-tagged biomolecules.
• Cell Surface Labeling & Imaging: Conjugation of biotin to cell surface proteins or other targets for visualization with fluorescent streptavidin probes.
• Protein-Protein Interaction Studies: Introduction of a biotin tag into one interaction partner for pull-down assays using streptavidin matrices.
• Nucleic Acid Probe Labeling: Biotinylation of oligonucleotides for use in hybridization-based detection methods (e.g., Southern blotting, in situ hybridization).
• Material Science & Surface Engineering: Creating biotin-functionalized surfaces for sensor development and biomaterial research.

Basic Information

Item	Details
Product Name	Pc-Biotin-Peg4-Nhs Carbonate
CAS No.	2055198-03-1
Molecular Formula	C33H48N6O12S
Molecular Weight	752.83 g/mol
Synonyms	Biotin-PEG4-NHS Carbonate; Biotin-PEG4-NHS Carbonate; PC Biotin PEG4 NHS Carbonate; Biotinamidohexanoyl-PEG4-NHS Carbonate; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(14-oxo-4,7,10-trioxa-13-azahexadecan-16-yl)pentanamide 2,5-Dioxopyrrolidin-1-yl Carbonate; Biotin-PEG4-NSC; Biotin-PEG4-SCM
EINECS	Contact for details

Quality Control

Our Pc-Biotin-Peg4-Nhs Carbonate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity confirmation and structural verification by NMR and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) with detailed specifications to ensure the product meets the exacting standards required for research and biopharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.