Description

n-(Propargyl-Peg4)-Biocytin is a high-purity, advanced biochemical reagent designed for precision conjugation and labeling applications. This compound is critical for researchers and developers requiring reliable, site-specific biotinylation of biomolecules via click chemistry. It is primarily utilized in the pharmaceutical, biotechnology, and life science research sectors for applications such as proteomics, biomarker discovery, and the development of targeted diagnostic and therapeutic agents.

Application

• Click Chemistry Conjugation: Acts as a versatile linker for the site-specific biotinylation of proteins, antibodies, peptides, and oligonucleotides via copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Proteomics & Protein Analysis: Used for labeling and enriching target proteins for pull-down assays, mass spectrometry analysis, and protein interaction studies.
• Diagnostic Assay Development: Enables the creation of sensitive detection platforms (e.g., ELISA, lateral flow assays) by conjugating biotin to detection molecules for streptavidin-based signal amplification.
• Drug Delivery & Targeting: Serves as a functional component in the synthesis of biotinylated drug conjugates and targeted nanoparticle systems.
• Cell Surface Labeling: Facilitates the tagging and visualization of cell surface receptors and other membrane proteins for flow cytometry and microscopy.
• Biomaterial Functionalization: Used to modify surfaces (e.g., chips, beads) with biotin for creating biosensor interfaces and affinity capture matrices.

Basic Information

Product Name	n-(Propargyl-Peg4)-Biocytin
CAS No.	2055042-71-0
Molecular Formula	C₃₃H₅₈N₆O₁₂S
Molecular Weight	750.91 g/mol
Synonyms	N-(Propargyl-PEG4)-Biocytin; Biotin-PEG4-Alkyne; Alkyne-PEG4-Biotin; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(prop-2-yn-1-yl)-N-(2-(2-(2-(2-(2-(2-(2-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)pentanamide; Propargyl-PEG4-Biocytin; Biocytin-PEG4-Alkyne; N-(Prop-2-yn-1-yl)-N-(2-(2-(2-(2-(2-(2-(2-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
EINECS	Contact for details

Quality Control

Our n-(Propargyl-Peg4)-Biocytin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification, NMR and MS for structural confirmation, and functional assay validation. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Assay	≥ 90% active

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.