Description

n-(Azido-Peg4)-Biocytin is a heterobifunctional crosslinking reagent that combines a biotin moiety with a tetraethylene glycol (PEG4) spacer and a terminal azide group. This molecular architecture is critical for enabling highly specific bioconjugation via click chemistry, primarily copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is an essential tool for researchers and developers in the life sciences and pharmaceutical industries who require precise, stable, and efficient labeling of biomolecules for detection, purification, and diagnostic applications.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of proteins, antibodies, and peptides for creating detection probes.
• Affinity Purification Probe Development: Synthesis of biotinylated ligands for streptavidin/avidin-based pull-down assays and purification systems.
• Cell Surface Engineering: Labeling of cell surface glycans or proteins for imaging, tracking, and sorting applications using metabolic labeling techniques.
• Diagnostic Assay Development: Conjugation of detection molecules in ELISA, lateral flow assays, and other immunoassay platforms.
• Chemical Biology and Proteomics: As a key reagent in activity-based protein profiling (ABPP) and other chemoproteomic strategies to study enzyme function.
• Nanoparticle Functionalization: Attachment of targeting or detection ligands to nanoparticles for therapeutic or diagnostic (theranostic) purposes.
• Material Science and Biointerface Engineering: Creating biofunctionalized surfaces for biosensors, microarrays, and tissue engineering scaffolds.

Basic Information

Product Name	n-(Azido-Peg4)-Biocytin
CAS No.	2055042-70-9
Molecular Formula	C₃₃H₆₁N₉O₁₀S
Molecular Weight	751.96 g/mol
Synonyms	Azido-PEG4-Biocytin; Biotin-PEG4-azide; N-(Azido-PEG4)-Biocytin; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(17-azido-3,6,9,12-tetraoxa-16-azaheptadecyl)pentanamide; Biocytin, N-[17-azido-3,6,9,12-tetraoxa-16-azaheptadecyl]-; Biotin-PEG4-N3; Azido-PEG4-Biotin analog; Biotinylated PEG azide linker
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg4)-Biocytin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and NMR/MS for structural confirmation, to ensure consistency and reliability for sensitive research applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.