Description

Mal-PEG8-NHS Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a maleimide group at one terminus and an N-hydroxysuccinimide (NHS) ester at the other. This compound is essential for creating stable, site-specific conjugations between thiol-containing molecules (like cysteine residues in proteins or peptides) and primary amine groups. It is a critical reagent for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced biomaterials engineering, enabling the precise construction of complex therapeutic and diagnostic agents.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein/Peptide PEGylation: Introduction of a hydrophilic PEG spacer to improve pharmacokinetics, solubility, and stability of biologics.
• Biomaterial Functionalization: Covalent attachment of bioactive peptides or proteins to hydrogel matrices, nanoparticles, or sensor surfaces.
• Diagnostic Probe Conjugation: Labeling of antibodies, proteins, or oligonucleotides with fluorophores, enzymes, or other detection moieties.
• Enzyme Immobilization: Stable, oriented coupling of enzymes to solid supports for biocatalysis and biosensing applications.
• Crosslinking for Structural Studies: Mapping protein-protein interactions or determining spatial relationships within macromolecular complexes.

Basic Information

Product Name	Mal-PEG8-NHS Ester
CAS No.	2055033-05-9
Molecular Formula	C33H56N2O15
Molecular Weight	720.8 g/mol
Synonyms	Maleimide-PEG8-NHS Ester; NHS-PEG8-Maleimide; α-Maleimide-ω-NHS-PEG8; N-(3-Maleimidopropionyl)-3,6,9,12,15,18,21,24-octaoxahexacosan-26-oic Acid Succinimidyl Ester; 3,6,9,12,15,18,21,24-Octaoxahexacosanedioic Acid, 26-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-1-[(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)methyl]-1-oxo-; Mal-PEG8-OSu; Maleimide-PEG8-NHS; PEG8 Spacer, Maleimide-NHS
EINECS	Contact for details

Quality Control

Our Mal-PEG8-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) are provided, detailing specifications for purity, identity, and functional group integrity. We support development under GMP guidelines and can supply material suitable for research and pre-clinical use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (H-NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (Maleimide)	≥ 90%
Functional Assay (NHS Ester)	≥ 90%
Solubility	Soluble in DMSO, DMF, acetonitrile, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.