Description

Sulfo Dbco-Maleimide CAS NO 2028281-86-7 is a heterobifunctional crosslinking agent engineered for advanced bioconjugation, combining a water-soluble sulfonated DBCO (dibenzocyclooctyne) group with a maleimide moiety. This dual functionality is critical for creating stable, site-specific linkages between biomolecules, enabling precise assembly of complex therapeutic and diagnostic constructs. It is an essential reagent for researchers and manufacturers in the fields of antibody-drug conjugate (ADC) development, protein engineering, and next-generation bioassay platforms.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: Enables controlled, click chemistry-based conjugation of cytotoxic payloads to antibodies via the DBCO-azide reaction, followed by maleimide-thiol coupling for linker attachment.
• Protein and Peptide Labeling: Facilitates the site-specific labeling of cysteine-containing proteins or engineered thiol groups with azide-modified fluorophores, biotin, or other probes.
• Surface Functionalization: Used to modify nanoparticles, biosensors, and microarray surfaces with biomolecules for enhanced targeting and detection capabilities.
• Polymer and Biomaterial Science: Serves as a key building block for creating functionalized hydrogels, drug delivery carriers, and smart materials with tailored biological activity.
• Diagnostic Assay Development: Critical for developing sensitive immunoassays and molecular diagnostics by creating stable conjugates between detection enzymes and antibodies or oligonucleotides.

Basic Information

Product Name	Sulfo Dbco-Maleimide
CAS No.	2028281-86-7
Molecular Formula	C₂₀H₁₅N₃O₇S₂ (Contact for exact confirmation)
Molecular Weight	~473.48 g/mol (Contact for exact confirmation)
Synonyms	DBCO-Sulfo-Maleimide; Sulfo-DBCO-MAL; 3,5-Dioxo-4-azaspiro[2.4]hept-6-yl 1-(11,12-didehydrodibenzo[b,f]azocin-5(6H)-yl)-3,6,9,12-tetraoxa-2-azatetradecan-14-oate 7,7-dioxide; 1-(3-(2-(2-(3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)propanamido)ethoxy)ethoxy)propyl)-3,5-dioxo-4-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-4-azaspiro[2.4]heptane-6-carboxylic acid; Sulfo-DBCO-PEG4-Maleimide; Water-soluble DBCO-Maleimide crosslinker.
EINECS	Contact for details

Quality Control

Our Sulfo Dbco-Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment to guarantee batch-to-batch consistency and support your regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.