Description

2,5-Dioxopyrrolidin-1-Yl 3-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Propanoate is a highly specialized, multifunctional chemical reagent designed for advanced bioconjugation and photoaffinity labeling applications. Its core value lies in the unique combination of an N-hydroxysuccinimide (NHS) ester for efficient amine coupling and a diazirine group for photo-activated, irreversible crosslinking upon UV irradiation. This compound is essential for researchers and developers in pharmaceutical discovery, proteomics, and chemical biology who require precise tools for mapping protein-protein interactions, identifying drug targets, and developing antibody-drug conjugates (ADCs).

Application

• Photoaffinity Labeling (PAL) Probes: Acts as a critical building block for synthesizing probes to capture transient or weak biomolecular interactions for target identification.
• Chemical Crosslinking Reagents: Used to create covalent bonds between biomolecules (e.g., proteins, nucleic acids) and other ligands or surfaces for structural studies and assay development.
• Proteomics Research: Enables the study of protein function, interaction networks, and post-translational modifications through selective labeling and enrichment strategies.
• Antibody-Drug Conjugate (ADC) Linker Development: Serves as a heterobifunctional linker precursor, connecting cytotoxic payloads to monoclonal antibodies via stable amide bonds and photo-crosslinkable sites.
• Surface Functionalization: Used to modify nanoparticles, biosensors, and microarray slides with photo-reactive groups for capturing specific biomolecules.
• Activity-Based Protein Profiling (ABPP): Facilitates the design of probes to monitor enzyme activity and selectivity within complex biological samples.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 3-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Propanoate
CAS No.	2012552-32-6
Molecular Formula	C13H15N3O4
Molecular Weight	277.28 g/mol
Synonyms	3-(3-(But-3-yn-1-yl)-3H-diazirin-3-yl)propanoic acid N-hydroxysuccinimide ester; NHS-Diazirine-Alkyne; Photo-reactive NHS ester; Heterobifunctional Crosslinker (NHS ester/Diazirine); Succinimidyl 3-(3-(but-3-yn-1-yl)diazirin-3-yl)propanoate; Alkyne-Diazirine-NHS Ester; Photo-leucine-based NHS ester; CAS 2012552-32-6
EINECS	Contact for details

Quality Control

Every batch of 2,5-Dioxopyrrolidin-1-Yl 3-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Propanoate is manufactured and tested under strict quality management systems. We guarantee high purity and batch-to-batch consistency critical for sensitive research applications. Comprehensive analytical data, including HPLC purity, NMR, and MS confirmation, are documented in the Certificate of Analysis (COA) provided with each shipment. Our quality commitment ensures the reagent performs reliably in your most demanding photoaffinity labeling and crosslinking experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment. This compound is strictly light-sensitive and hygroscopic. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store as recommended and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.