Description

4-Methylumbelliferyl 6-o-(tert-Butyldiphenylsilyl)-β-D-Galactopyranoside is a specialized fluorogenic substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of β-galactosidase activity. It is primarily utilized in the biotechnology, pharmaceutical, and diagnostic industries for applications ranging from gene expression reporting to enzyme-linked immunosorbent assays (ELISA).

Application

• Fluorogenic Substrate for β-Galactosidase: Used as a highly sensitive probe in enzymatic assays to detect and quantify β-galactosidase activity, commonly employed as a reporter gene in molecular biology.
• Diagnostic Kit Development: A key component in the formulation of diagnostic kits for detecting bacterial contamination (e.g., E. coli) and certain genetic disorders linked to enzyme deficiencies.
• High-Throughput Screening (HTS): Enables rapid, fluorescent-based screening of compound libraries in drug discovery pipelines targeting glycosidase enzymes.
• Cell Biology & Imaging: Facilitates the visualization of gene expression and cellular localization in live-cell and fixed-cell imaging studies.
• Biochemical Research: Serves as a critical tool for studying enzyme kinetics, inhibition, and the mechanism of action of β-galactosidase and related enzymes.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme preparations and recombinant proteins in biopharmaceutical production.

Basic Information

Item	Detail
Product Name	4-Methylumbelliferyl 6-o-(tert-Butyldiphenylsilyl)-β-D-Galactopyranoside
CAS No.	296776-03-9
Molecular Formula	C32H36O9Si
Molecular Weight	592.72 g/mol
Synonyms	4-MU-6-O-TBDPS-β-D-Galactopyranoside; 4-Methylumbelliferyl 6-O-(tert-butyldiphenylsilyl)-β-D-galactopyranoside; 6-O-TBDPS-4-Methylumbelliferyl-β-D-galactopyranoside; TBDPS-4-MU-β-Gal; Fluorogenic β-galactosidase substrate (TBDPS protected); 4-Methylumbelliferyl β-D-galactopyranoside 6-tert-butyldiphenylsilyl ether
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl 6-o-(tert-Butyldiphenylsilyl)-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for research and diagnostic applications. All products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider storing under an inert atmosphere to maximize stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details
Fluorogenic Activity	Functional in standard β-galactosidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.