Description

Ac-Leu-Glu-His-Asp-Amc CAS NO 292633-16-0 is a fluorogenic peptide substrate designed for the detection and quantification of specific protease activity. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of enzymatic kinetics. It is primarily needed by researchers and quality control laboratories in the life sciences, pharmaceutical, and biotechnology industries for high-throughput screening and diagnostic assay development.

Application

• Protease Activity Assays: Used as a specific fluorogenic substrate for measuring the activity of caspases and other related proteases in apoptosis research.
• High-Throughput Screening (HTS): Enables rapid screening of potential drug candidates that modulate protease activity in pharmaceutical discovery.
• Biochemical Research: Serves as a key tool for studying enzyme kinetics, inhibition mechanisms, and substrate specificity in vitro.
• Diagnostic Development: Utilized in the development of sensitive fluorometric assays for clinical diagnostics and biomarker detection.
• Cell Biology Studies: Applied in live-cell or lysate-based assays to monitor protease activation during cellular processes.
• Quality Control Testing: Employed in biopharmaceutical manufacturing to test the activity and consistency of enzyme-based products.

Basic Information

Product Name	Ac-Leu-Glu-His-Asp-Amc
CAS No.	292633-16-0
Molecular Formula	C33H45N9O12
Molecular Weight	735.77 g/mol
Synonyms	Ac-LEHD-AFC; Ac-Leu-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin; Acetyl-Leu-Glu-His-Asp-AFC; Caspase-9 Substrate I (Ac-LEHD-AFC); Ac-LEHD-AFC Caspase-9 Fluorogenic Substrate; N-Acetyl-L-leucyl-L-α-glutamyl-L-histidyl-L-α-aspartyl-7-amino-4-trifluoromethylcoumarin; LEHDase Substrate; AFC-Labeled Caspase Substrate
EINECS	Contact for details

Quality Control

Our Ac-Leu-Glu-His-Asp-Amc is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to relevant industry guidelines to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF
Functional Activity	Conforms to reference standard in caspase-9 assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.