Description

Mca-Glu-Asp-Ala-Ser-Thr-Pro-Cys-Oh (CAS NO 291297-58-0) is a synthetic peptide derivative featuring a 7-amino acid sequence with a 7-methoxycoumarin-4-acetyl (Mca) fluorophore at the N-terminus. This compound is primarily valued as a highly specific and sensitive fluorogenic substrate for enzymatic activity assays, particularly for proteases. It is an essential research tool for scientists in pharmaceutical development, biochemistry, and molecular biology who require precise measurement of enzyme kinetics and inhibition.

Application

• Protease Research & Drug Discovery: Acts as a fluorogenic substrate for studying the activity, specificity, and inhibition of various proteolytic enzymes, including caspases, cathepsins, and other serine or cysteine proteases.
• High-Throughput Screening (HTS): Enables the rapid screening of compound libraries for potential protease inhibitors in automated assay systems for drug candidate identification.
• Biochemical Assay Development: Used in the design and optimization of sensitive in vitro diagnostic assays to measure protease activity in biological samples.
• Apoptosis (Programmed Cell Death) Studies: A critical tool for monitoring caspase activity, which is a key indicator and mediator of apoptotic pathways in cellular research.
• Enzyme Kinetics Characterization: Provides precise data for determining kinetic parameters (Km, Vmax, kcat) of proteases due to its cleavable peptide bond and fluorescent signal release.
• Quality Control in Biologics Manufacturing: Can be utilized in downstream process development to monitor the presence and activity of specific proteases.

Basic Information

Product Name	Mca-Glu-Asp-Ala-Ser-Thr-Pro-Cys-Oh
CAS No.	291297-58-0
Molecular Formula	C41H53N9O18S
Molecular Weight	967.97 g/mol
Synonyms	7-Methoxycoumarin-4-acetyl-Glu-Asp-Ala-Ser-Thr-Pro-Cys-OH; Mca-Glu-Asp-Ala-Ser-Thr-Pro-Cys-OH; Caspase-3 Substrate I (Mca-DEVDAPK(Dnp)-OH related); Fluorogenic Caspase-3 Substrate; Mca-DEVDAPK(Dnp)-OH Analog; (7-Methoxycoumarin-4-yl)acetyl-Glu-Asp-Ala-Ser-Thr-Pro-Cys-OH; Ac-Mca-Glu-Asp-Ala-Ser-Thr-Pro-Cys-OH
EINECS	Contact for details

Quality Control

Our Mca-Glu-Asp-Ala-Ser-Thr-Pro-Cys-Oh is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are provided to guarantee the material meets the stringent requirements for life science research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and easily oxidized; it is recommended to store under an inert atmosphere such as argon or nitrogen after opening. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.