Description

4-Diphosphocytidyl-2-C-Methyl-D-Erythritol (Cdp-Me) is a key nucleotide-activated intermediate in the non-mevalonate (MEP/DOXP) pathway for isoprenoid biosynthesis. This compound matters significantly for research and development in metabolic engineering and antimicrobial drug discovery, as it is a precursor to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). It is primarily needed by biotechnology firms, academic research institutions, and pharmaceutical companies focusing on novel antibiotic targets, particularly against pathogens like *Mycobacterium tuberculosis* and *Plasmodium falciparum*.

Application

• Biochemical Research: Critical substrate for studying the enzymes of the MEP pathway, such as IspD and IspE.
• Drug Discovery: Target molecule for the development of novel antibiotics and antimalarials that inhibit bacterial and apicomplexan isoprenoid biosynthesis.
• Metabolic Engineering: Used in microbial systems engineered for the high-yield production of isoprenoids, such as terpenes and carotenoids.
• Enzyme Kinetics & Inhibition Studies: Serves as a standard and inhibitor for characterizing enzyme activity and mechanism.
• Diagnostic Reagent Development: Potential use in assays to screen for compounds that disrupt essential microbial metabolic pathways.
• Synthetic Biology: Building block for reconstructing and optimizing the MEP pathway in heterologous hosts.

Basic Information

Product Name	4-Diphosphocytidyl-2-C-Methyl-D-Erythritol (Cdp-Me)
CAS No.	263016-94-0
Molecular Formula	C15H27N3O16P3
Molecular Weight	598.29 g/mol
Synonyms	CDP-ME; 2-C-Methyl-D-erythritol 4-cytidylyl diphosphate; 2-C-Methyl-D-erythritol 4-cytidine 5'-diphosphate; Cdp-2-C-methyl-D-erythritol; 4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol; MEP pathway intermediate; 4-(5'-O-Phosphonocytidylyl)-2-C-methyl-D-erythritol diphosphate
EINECS	Contact for details

Quality Control

Our 4-Diphosphocytidyl-2-C-Methyl-D-Erythritol is produced under stringent conditions suitable for research applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all critical quality parameters to ensure reproducibility and reliability for your sensitive biochemical assays and research projects.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated under an inert atmosphere. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.