Description

4-Nitrophenyl 2-Acetamido-2-Deoxy-4-o-A-L-Fucopyranosyl-B-D-Glucopyranoside is a specialized chromogenic substrate designed for the sensitive detection and kinetic analysis of specific glycosidase enzymes. This compound matters as a critical research tool, enabling precise biochemical assays in enzymology and glycobiology by releasing a colored 4-nitrophenol product upon enzymatic cleavage. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors for studying enzyme mechanisms, screening inhibitors, and developing analytical methods.

Application

• Glycosidase Enzyme Assays: Used as a chromogenic substrate for the detection and kinetic characterization of specific α-L-fucosidases and related enzymes.
• Biochemical Research: A key tool in glycobiology for studying carbohydrate-active enzymes, substrate specificity, and enzymatic pathways.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential glycosidase inhibitors for drug discovery.
• Diagnostic Development: Serves as a core component in the development of clinical diagnostic kits for enzyme deficiency disorders.
• Quality Control: Used in biomanufacturing to monitor enzyme activity and ensure batch-to-batch consistency of enzymatic products.

Basic Information

Product Name	4-Nitrophenyl 2-Acetamido-2-Deoxy-4-o-A-L-Fucopyranosyl-B-D-Glucopyranoside
CAS No.	259143-52-7
Molecular Formula	C26H36N2O15
Molecular Weight	616.57 g/mol
Synonyms	p-Nitrophenyl α-L-fucopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside; 4-Nitrophenyl N-acetyl-β-D-lactosaminide analog; Fucosylated chromogenic substrate; pNP-α-L-Fuc-(1→4)-β-D-GlcNAc; Glycosidase substrate 259143-52-7; 4-Nitrophenyl 2-acetamido-2-deoxy-4-O-α-L-fucopyranosyl-β-D-glucopyranoside; α-L-Fuc(1-4)β-D-GlcNAc-pNP
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 2-Acetamido-2-Deoxy-4-o-A-L-Fucopyranosyl-B-D-Glucopyranoside is produced under strict quality management protocols to ensure high purity and batch-to-batch reproducibility suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.