Description

N4-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-3'-o-(2-Methoxyethyl)-5-Methylcytidine is a sophisticated, protected nucleoside derivative essential for the synthesis of oligonucleotides. This compound matters because it provides critical protection for the cytosine base and ribose sugar during solid-phase synthesis, ensuring high coupling efficiency and product purity. It is primarily needed by research institutions and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids. Our global supply ensures consistent quality and reliable availability for your most demanding synthesis projects.

Application

• Solid-Phase Oligonucleotide Synthesis: A key phosphoramidite building block for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Critical raw material in the production of antisense drugs, siRNA, and aptamers for clinical development.
• Research-Grade Probe Synthesis: Used to manufacture labeled and modified probes for genetic analysis, diagnostics, and basic research.
• Synthesis of Modified Nucleosides: Serves as a versatile intermediate for creating nucleoside analogs with tailored properties.
• Scale-Up Process Development: Supports process optimization and cGMP manufacturing for oligonucleotide-based therapeutics.

Basic Information

Product Name	N4-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-3'-o-(2-Methoxyethyl)-5-Methylcytidine
CAS No.	256223-98-0
Molecular Formula	C46H49N3O9
Molecular Weight	787.90 g/mol
Synonyms	5-Methylcytidine, N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-O-(2-methoxyethyl)-; 5'-O-DMT-3'-O-(2-methoxyethyl)-N4-benzoyl-5-methylcytidine; 3'-O-(2-Methoxyethyl)-5-methylcytidine DMT-protected; DMT-2'-MOE-5-methylcytidine, N4-Bz-protected; 5-Me-C(Bz) 2'-MOE-CE Phosphoramidite precursor; 2'-O-Methoxyethyl-5-methylcytidine derivative; Protected 5-Methylcytidine for oligonucleotide synthesis
EINECS	Contact for details

Quality Control

Every batch of N4-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-3'-o-(2-Methoxyethyl)-5-Methylcytidine is manufactured and tested under strict quality systems. We ensure compliance with the high-purity standards required for pharmaceutical research and development. Comprehensive analytical testing, including HPLC, NMR, and mass spectrometry, is performed to verify identity, purity, and stability. A Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.