Description

Hexokinase from Yeast is a highly purified enzyme that catalyzes the phosphorylation of hexose sugars, a critical first step in glycolysis. This product is essential for research and industrial applications requiring precise, reliable enzymatic activity. It is primarily used by biotech, pharmaceutical, and diagnostic industries for biochemical assays, diagnostic reagent formulation, and metabolic pathway studies.

Application

• Diagnostic Reagent Manufacturing: A key component in clinical chemistry kits for the quantitative determination of glucose, ATP, and other metabolites in blood serum.
• Biochemical Research: Used in enzymatic assays and metabolic studies to investigate glycolysis, kinase activity, and energy metabolism pathways.
• Pharmaceutical Development: Employed in screening platforms for drug discovery targeting metabolic disorders and cancer metabolism.
• Food & Beverage Analysis: Applied in enzymatic methods for the accurate measurement of sugar content and fermentation process monitoring.
• Enzyme Cascade Reactions: Integrated into multi-enzyme systems for the synthesis of high-value biochemicals and biosensors.

Basic Information

Product Name	Hexokinase from Yeast
CAS No.	254866-44-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	ATP:D-hexose 6-phosphotransferase; Yeast Hexokinase; HK; Hexokinase Type F-300; Hexokinase (Yeast); D-hexose-6-phosphate:ATP phosphotransferase; Glucokinase (non-specific); Hexokinase PII; EC 2.7.1.1
EINECS	Contact for details

Quality Control

Our Hexokinase from Yeast is produced and tested under strict quality management systems. Each lot undergoes rigorous analysis to ensure high specific activity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing assay results, activity units, and purity is provided with every shipment to guarantee compliance with your research and manufacturing standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ 100 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Contact for details
Stabilizers	May contain glycerol or salts
Activity Definition	One unit will phosphorylate 1.0 μmole of D-glucose to D-glucose 6-phosphate per minute at pH 8.0 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.