Description

4-Methylumbelliferyl-B-D-Xylotrioside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of specific enzymatic activity. This compound is critical for researchers and quality control laboratories requiring precise, high-throughput biochemical assays. It is primarily utilized in the life sciences, pharmaceutical research, and diagnostic development sectors for studying glycosidase enzymes, particularly in carbohydrate metabolism research and enzyme inhibitor screening.

Application

• Glycosidase Enzyme Assays: A key fluorogenic substrate for the continuous, sensitive detection of specific endo-xylanase and other glycosidase activities.
• Biochemical Research: Used in fundamental research on carbohydrate-active enzymes (CAZymes), enzyme kinetics, and mechanism-of-action studies.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for therapeutic development.
• Diagnostic Development: Serves as a critical reagent in developing clinical diagnostic kits for enzyme deficiency disorders.
• Industrial Enzyme Characterization: Used to assay and optimize enzyme preparations used in biofuel, pulp/paper, and food processing industries.
• Quality Control (QC) Testing: Provides a standardized method for the QC of enzyme products and related biochemical reagents.

Basic Information

Product Name	4-Methylumbelliferyl-B-D-Xylotrioside
CAS No.	250252-60-9
Molecular Formula	C26H34O16
Molecular Weight	602.54 g/mol
Synonyms	4-MU-β-D-Xylotrioside; 4-Methylumbelliferyl β-D-xylotrioside; 4-MU-Xylotriose; 4-Methylumbelliferyl xylotrioside; MUX3; Fluorogenic xylotrioside substrate; 4-MU-β-D-Xylotriose; 4-Methylumbelliferyl-β-D-xylotriose
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-B-D-Xylotrioside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Fluorogenic Activity	Conforms (Functional assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.