Description

(1-Acetyl-2,2,5,5-Tetramethyl--Pyrroline-3-Methyl) Methanethiosulfonate is a specialized spin-labeling reagent, also known as MTSSL, widely used in site-directed spin labeling (SDSL) for electron paramagnetic resonance (EPR) spectroscopy. Its core value lies in enabling the study of protein dynamics, conformational changes, and structural mapping in complex biological systems. This compound is essential for researchers and developers in structural biology, biophysics, and pharmaceutical discovery who require precise tools for probing macromolecular structure and function.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Primary application for covalent attachment of a stable nitroxide radical to cysteine residues in proteins and nucleic acids.
• Protein Dynamics and Folding Studies: Used to investigate conformational changes, flexibility, and folding pathways of biomolecules.
• Membrane Protein Research: Critical for labeling and studying the structure and dynamics of integral membrane proteins.
• Distance Measurements (DEER/PELDOR): Enables precise nanometer-scale distance measurements between spin labels to determine macromolecular architecture.
• Drug Discovery and Biologics Development: Supports the characterization of protein-ligand interactions and the stability of therapeutic proteins.
• Structural Biology Tool: A key reagent for complementing techniques like X-ray crystallography and cryo-EM with dynamic information.

Basic Information

Product Name	(1-Acetyl-2,2,5,5-Tetramethyl--Pyrroline-3-Methyl) Methanethiosulfonate
CAS No.	244641-23-4
Molecular Formula	C12H20N2O3S2
Molecular Weight	304.43 g/mol
Synonyms	MTSSL; 1-Oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-methyl methanethiosulfonate; (1-Acetyl-2,2,5,5-tetramethyl-3-pyrrolin-3-yl)methyl methanesulfonothioate; S-(1-Acetyl-2,2,5,5-tetramethyl-3-pyrrolin-3-ylmethyl) methanethiosulfonate; Methanethiosulfonic acid S-[(1-acetyl-2,2,5,5-tetramethyl-3-pyrrolin-3-yl)methyl] ester; Spin label MTSSL; Nitroxide spin label reagent
EINECS	Contact for details

Quality Control

Our (1-Acetyl-2,2,5,5-Tetramethyl--Pyrroline-3-Methyl) Methanethiosulfonate is produced under strict quality management systems to ensure high purity and batch-to-batch consistency, critical for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and spectroscopic methods for identity confirmation. Certificates of Analysis (COA) with detailed specifications are provided to guarantee the product meets the rigorous standards required for EPR spectroscopy and structural biology research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is light-sensitive (store away from light) and should be handled under anhydrous conditions to prevent degradation. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.