Description

Boc-Thiono-T-Leu-1-(6-Nitro)Benzotriazolide is a specialized, high-purity protected amino acid derivative designed for advanced peptide synthesis and medicinal chemistry research. This compound serves as a critical building block for the controlled introduction of thionated leucine analogs, enabling the study of peptide structure-activity relationships and the development of novel therapeutic agents. It is primarily utilized by research scientists and process chemists in pharmaceutical R&D, biotechnology, and academic institutions focused on peptide-based drug discovery and chemical biology.

Application

• Solid-Phase Peptide Synthesis (SPPS): Used as an activated ester reagent for the efficient, racemization-free incorporation of thionated leucine residues into peptide chains.
• Medicinal Chemistry & Drug Discovery: A key intermediate in the synthesis of peptide mimetics and protease inhibitors, where the thiono group modulates bioactivity and stability.
• Chemical Biology Probes: Enables the creation of labeled or modified peptides for studying protein-protein interactions and enzyme mechanisms.
• Process Development: Serves as a starting material or intermediate in the scalable synthesis of active pharmaceutical ingredients (APIs) containing non-standard amino acids.
• Academic Research: Facilitates studies in peptide science, including the exploration of novel secondary structures and the physicochemical properties of thioamide bonds.

Basic Information

Product Name	Boc-Thiono-T-Leu-1-(6-Nitro)Benzotriazolide
CAS No.	242483-79-0
Molecular Formula	C20H27N5O5S
Molecular Weight	449.52 g/mol
Synonyms	1-[(6-Nitro-1H-benzotriazol-1-yl)oxy]-2,5-pyrrolidinedione, 3-[(1,1-Dimethylethoxy)carbonyl]-4-methyl-2-thioxopentyl ester; Boc-Thio-Leu-OBt; tert-Butyloxycarbonyl-thionoleucine-6-nitrobenzotriazolyl ester; Boc-L-thionoleucine 6-nitrobenzotriazolide; (S)-2-((tert-Butoxycarbonyl)amino)-4-methyl-1-thioxopentan-1-yl 6-nitro-1H-benzo[d][1,2,3]triazole-1-carboxylate; Thionated Boc-Leu-ONb; Boc-Thio-Leu-ONb
EINECS	Contact for details

Quality Control

Our Boc-Thiono-T-Leu-1-(6-Nitro)Benzotriazolide is manufactured under controlled conditions to ensure high chemical purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent decomposition. For long-term storage, consider desiccant and under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.