Description

p-Nitrophenyl 2-Acetamido-2-Deoxy-(6-o-2-Acetamido-2-Deoxy-B-D-Glucopyranosyl)-A-D-Galactopyranoside is a specialized chromogenic substrate designed for the sensitive and specific detection of glycosidase enzyme activity. This compound matters for its critical role in biochemical research and diagnostic development, where precise enzyme kinetics are essential. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for studying carbohydrate-active enzymes.

Application

• Enzyme Assay Substrate: A chromogenic substrate for the detection and kinetic analysis of specific glycosidases, including β-N-acetylhexosaminidases and related enzymes.
• Biochemical Research: Used in fundamental research to study enzyme mechanisms, inhibition, and substrate specificity within carbohydrate metabolism pathways.
• Diagnostic Reagent Development: Employed in the formulation of diagnostic kits for the detection of enzyme deficiencies or specific biomarkers in clinical samples.
• High-Throughput Screening (HTS): Utilized in pharmaceutical and biotechnology labs for screening compound libraries for potential glycosidase inhibitors as drug candidates.
• Quality Control in Biomanufacturing: Applied in monitoring enzyme activity during the production and purification of recombinant glycosidases.
• Microbiology Studies: Used to characterize and identify bacterial strains based on their specific glycosidase activity profiles.

Basic Information

Product Name	p-Nitrophenyl 2-Acetamido-2-Deoxy-(6-o-2-Acetamido-2-Deoxy-B-D-Glucopyranosyl)-A-D-Galactopyranoside
CAS No.	235752-73-5
Molecular Formula	C28H39N3O16
Molecular Weight	673.62 g/mol
Synonyms	pNP-β-D-GlcNAc-(1→6)-α-D-GalNAc; p-Nitrophenyl N,N'-diacetyl-β-chitobioside; 4-Nitrophenyl 2-acetamido-2-deoxy-6-O-[2-acetamido-2-deoxy-β-D-glucopyranosyl]-α-D-galactopyranoside; pNP-β-D-GlcNAc-(1→6)-α-D-GalNAc-pNP; Chromogenic substrate for β-N-acetylhexosaminidase; N,N'-Diacetylchitobioside p-nitrophenyl derivative; 4-NP-β-GlcNAc-(1,6)-α-GalNAc
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 2-Acetamido-2-Deoxy-(6-o-2-Acetamido-2-Deoxy-B-D-Glucopyranosyl)-A-D-Galactopyranoside is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. Due to its hygroscopic nature, the container should be kept sealed under desiccant when not in use to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.