Description

o-Nitrophenyl β-D-Galactopyranoside-6-Phosphate, Cyclohexylammonium Salt is a specialized chromogenic substrate designed for the detection and quantification of enzymatic activity. This compound is critical for research and diagnostic applications requiring high sensitivity and specificity. It is primarily utilized by biotech and pharmaceutical R&D teams, as well as diagnostic kit manufacturers, for enzyme-linked assays and molecular biology research.

Application

• Enzyme Substrate for Diagnostics: A key chromogenic reagent for detecting β-galactosidase activity in ELISA, histochemistry, and microbiological assays.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ gene expression) to study gene regulation and promoter activity.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify enzyme inhibitors or activators for drug discovery.
• Biochemical Reagent: Serves as a critical component in buffer systems and kits for the precise measurement of enzymatic kinetics.
• Microbiology: Applied in microbial identification and studies of bacterial gene expression where β-galactosidase is a marker.
• Quality Control Testing: Used in the quality assurance of therapeutic proteins and other biologics that utilize enzyme tags.

Basic Information

Product Name	o-Nitrophenyl β-D-Galactopyranoside-6-Phosphate, Cyclohexylammonium Salt
CAS No.	225917-39-5
Molecular Formula	C18H29N2O13P
Molecular Weight	512.41 g/mol
Synonyms	ONPG-6-Phosphate, Cyclohexylammonium Salt; 2-Nitrophenyl β-D-galactopyranoside 6-(dihydrogen phosphate) cyclohexylammonium salt; o-Nitrophenyl-β-D-galactopyranoside-6-phosphate; ONPGP; 6-Phosphorylated ONPG; Galactosidase substrate-6-phosphate; β-Galactosidase chromogenic substrate phosphate ester
EINECS	Contact for details

Quality Control

Our o-Nitrophenyl β-D-Galactopyranoside-6-Phosphate, Cyclohexylammonium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO
Specific Rotation	Contact for details
Extinction Coefficient	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.