Description

4-Methylumbelliferyl2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranoside is a specialized fluorogenic glycosidase substrate used for the sensitive detection and quantification of enzyme activity. This compound matters as a critical research tool for studying carbohydrate metabolism, specifically for enzymes like α-L-fucosidase and β-galactosidase. It is primarily needed by biochemical researchers, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme inhibitor screening and lysosomal storage disorder research.

Application

• Enzyme Activity Assays: A key fluorogenic substrate for the continuous, high-sensitivity measurement of α-L-fucosidase activity in research and clinical diagnostics.
• Diagnostic Reagent Manufacturing: Used in the formulation of kits for the diagnosis of genetic disorders such as fucosidosis.
• Drug Discovery & High-Throughput Screening (HTS): Employed in pharmaceutical research to identify and characterize novel glycosidase inhibitors.
• Biochemical Research: Essential for studying carbohydrate-processing enzymes, glycobiology, and lysosomal function in cell culture and tissue samples.
• Microbiology: Can be used to study bacterial glycosidases for microbiological identification and research.
• Quality Control in Biologics: Acts as a process control reagent in the production and purification of therapeutic enzymes.

Basic Information

Product Name	4-Methylumbelliferyl2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranoside
CAS No.	225217-42-5
Molecular Formula	C22H28O13
Molecular Weight	500.45 g/mol
Synonyms	4-MU-α-L-Fucopyranosyl-(1→2)-β-D-galactopyranoside; 4-Methylumbelliferyl α-L-Fucopyranosyl-(1→2)-β-D-Galactopyranoside; 4-MU-Fuc-α(1-2)-Gal-β; Fucosidase Substrate; α-L-Fucosidase Fluorogenic Substrate; 4-Methylumbelliferyl-2'-fucosyllactoside; 4-MU-2'-FL
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranoside is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, typically ≥98% (HPLC). Each lot undergoes comprehensive analytical testing including HPLC for purity and assay, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, Methanol, Water
Fluorescence	Emits blue fluorescence upon enzymatic cleavage (λex ~365 nm, λem ~450 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.