Description

6-n-Biotinylaminohexyl Isopropyl Hydrogenphosphonate is a high-purity, functionalized biotin derivative designed for advanced bioconjugation and molecular labeling applications. This compound is critical for researchers and manufacturers requiring precise, covalent attachment of biotin tags to biomolecules, enabling downstream detection, purification, and analysis. It is primarily utilized by the life sciences, pharmaceutical, and diagnostic industries for developing sensitive assays, targeted drug delivery systems, and affinity-based separation platforms.

Application

• Bioconjugation Reagent: For the site-specific labeling of proteins, antibodies, peptides, and oligonucleotides with biotin.
• Affinity Chromatography Ligand Synthesis: Used to functionalize solid supports (e.g., agarose, magnetic beads) for streptavidin/avidin-based purification systems.
• Diagnostic Assay Development: A key component in the manufacture of ELISA, lateral flow assays, and other immunoassays requiring high-sensitivity detection.
• Drug Delivery & Targeting: Facilitates the creation of biotinylated drug conjugates and targeting moieties for enhanced cellular uptake.
• Molecular Probes & Imaging Agents: Serves as a precursor for synthesizing biotin-labeled probes used in fluorescence microscopy, flow cytometry, and in vivo imaging.
• Surface Plasmon Resonance (SPR) & Biosensors: Used to immobilize biomolecules on sensor chips for real-time interaction analysis.
• Pull-Down Assays & Proteomics: Essential for biotinylating bait proteins to isolate interacting partners from complex biological mixtures.
• Nucleic Acid Labeling: Enables the introduction of biotin tags into DNA or RNA sequences for capture and detection applications.

Basic Information

Product Name	6-n-Biotinylaminohexyl Isopropyl Hydrogenphosphonate
CAS No.	224583-37-3
Molecular Formula	C20H40N4O5PS
Molecular Weight	488.59 g/mol
Synonyms	Biotin-6-aminohexyl isopropyl phosphonate; Biotinamidohexyl isopropyl hydrogenphosphonate; N-Biotinyl-6-aminohexyl isopropyl phosphonate; Isopropyl 6-(biotinamido)hexyl hydrogenphosphonate; Biotin-PEG6-isopropylphosphonate; Biotinylated aminohexyl phosphonate; Biotin-ACP (Aminohexyl-C3-Phosphonate); 6-(Biotinamido)hexyl isopropylphosphonate
EINECS	Contact for details

Quality Control

Our 6-n-Biotinylaminohexyl Isopropyl Hydrogenphosphonate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for moisture and residual solvents, to ensure it meets the high standards required for research and biopharmaceutical applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.