Description

D-Luciferin 1-(4,5-Dimethoxy-2-Nitrophenyl)Ethyl Ester is a specialized, caged luciferin derivative designed for precise control in bioluminescence imaging and assay systems. This compound matters because it serves as a photolabile, inactive precursor that can be rapidly activated by UV light to release active D-luciferin, enabling spatiotemporal control over luminescent signal generation in live cells and organisms. Researchers in pharmaceutical discovery, molecular biology, and bioimaging need it for advanced applications such as real-time monitoring of gene expression, high-throughput screening (HTS), and studying dynamic cellular processes with minimal background interference.

Application

• Real-Time Bioluminescence Imaging (BLI): For non-invasive, longitudinal tracking of tumor growth, metastasis, and therapeutic response in live animal models.
• Controlled Gene Expression Studies: Enables precise, light-triggered activation of luciferase reporter systems to study promoter activity and cellular signaling pathways with high temporal resolution.
• High-Throughput Screening (HTS) Assays: Used in cell-based assays for drug discovery, allowing uncaging of the substrate at a defined time point to initiate the luminescent reaction.
• Cellular and Subcellular Process Analysis: Facilitates the study of fast biological events, such as protein-protein interactions or ion flux, by providing a rapid "turn-on" signal upon photoactivation.
• Development of Advanced Biosensors: A key component in the design of caged probe systems for detecting specific enzymes, metabolites, or other analytes in complex biological samples.

Basic Information

Product Name	D-Luciferin 1-(4,5-Dimethoxy-2-Nitrophenyl)Ethyl Ester
CAS No.	223920-67-0
Molecular Formula	C₂₂H₂₂N₂O₈S
Molecular Weight	474.49 g/mol
Synonyms	DMNPE-Caged D-Luciferin; D-Luciferin, 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester; Caged D-Luciferin; Photocaged Luciferin; 1-(4,5-Dimethoxy-2-nitrophenyl)ethyl D-luciferin ester; 4,5-Dimethoxy-2-nitrobenzyl (DMNB) caged luciferin; (4,5-Dimethoxy-2-nitrobenzyl) Luciferin; Substrate for controlled bioluminescence.
EINECS	Contact for details

Quality Control

Our D-Luciferin 1-(4,5-Dimethoxy-2-Nitrophenyl)Ethyl Ester is produced under strict quality management protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. Certificates of Analysis (COA) detailing purity, identity, and related substances are available upon request to support your regulatory and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. This material is strictly light-sensitive (protect from all light exposure). For long-term stability, store at -20°C or below under desiccated conditions. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Keep the container in a dry, cool, and well-ventilated area.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.