Description

H-Pro-Leu-Gly-Pna CAS NO 219138-16-6 is a high-purity synthetic peptide derivative, specifically a chromogenic substrate designed for enzymatic activity studies. This compound is critical for biochemical research and pharmaceutical development, providing a reliable and sensitive tool for protease and peptidase assay development. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics, inhibitor screening, and proteolytic pathway analysis.

Application

• Biochemical Research: As a chromogenic p-nitroanilide (Pna) substrate for studying the specificity and kinetics of proline-specific enzymes like dipeptidyl peptidases (DPPs).
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and characterize novel inhibitors for therapeutic targets in metabolic and inflammatory diseases.
• Diagnostic Development: Serves as a key component in the formulation of diagnostic assays for measuring specific enzyme activities in clinical samples.
• Enzyme Characterization: Essential for determining kinetic parameters (Km, Vmax) and elucidating the catalytic mechanism of proteases.
• Quality Control in Biologics: Employed in QC labs to monitor the activity and stability of enzyme-based biopharmaceutical products.

Basic Information

Product Name	H-Pro-Leu-Gly-Pna
CAS No.	219138-16-6
Molecular Formula	C20H28N6O5
Molecular Weight	432.48 g/mol
Synonyms	H-Pro-Leu-Gly-pNA; Pro-Leu-Gly-p-Nitroanilide; Prolyl-Leucyl-Glycine p-nitroanilide; DPP Substrate; DPP IV Substrate H-Pro-Leu-Gly-pNA; Chromogenic Substrate for DPP; Peptide p-nitroanilide substrate; 219138-16-6
EINECS	Contact for details

Quality Control

Every batch of H-Pro-Leu-Gly-Pna is manufactured under strict quality management protocols. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	95.0% - 105.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.