Description

H-Asp(Obzl)-Amc Hcl CAS NO 219138-15-5 is a protected aspartic acid derivative, specifically the benzyl ester of aspartic acid coupled with 7-amino-4-methylcoumarin (AMC) and supplied as the hydrochloride salt. This compound is a critical fluorogenic substrate and a key synthetic intermediate in peptide chemistry and protease research. It is primarily utilized by research scientists and process developers in the pharmaceutical and biotechnology sectors for enzyme kinetics studies, high-throughput screening assays, and the synthesis of more complex peptide-based molecules.

Application

• Protease & Enzyme Research: Acts as a sensitive fluorogenic substrate for studying the activity, kinetics, and inhibition of various aspartic proteases and other hydrolytic enzymes.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Peptide Synthesis: Serves as a key protected building block (Asp(OBzl)) in solid-phase and solution-phase peptide synthesis (SPPS).
• Biochemical Assay Development: Used to develop and optimize diagnostic and research assays that rely on enzymatic cleavage and fluorescence detection.
• Chemical Biology Probes: Incorporated into activity-based probes (ABPs) for profiling enzyme function in complex biological systems.

Basic Information

Product Name	H-Asp(Obzl)-Amc Hcl
CAS No.	219138-15-5
Molecular Formula	C22H23N3O5 • HCl
Molecular Weight	445.90 g/mol (Free base: 409.44 g/mol)
Synonyms	H-Asp(OBzl)-AMC • HCl; Aspartic acid β-benzyl ester-AMC hydrochloride; L-Aspartic acid 4-benzyl ester-7-amido-4-methylcoumarin hydrochloride; Asp(OBzl)-AMC; H-Asp(OBzl)-AMC hydrochloride; N-[(Benzyloxy)carbonyl]-L-aspartic acid α-7-amido-4-methylcoumarin hydrochloride; Fluorogenic substrate for aspartic proteases; Z-Asp(OBzl)-AMC (common naming variant)
EINECS	Contact for details

Quality Control

Our H-Asp(Obzl)-Amc Hcl is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.