Description

H-Asp-Amc is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of enzymatic activity. It is primarily utilized by researchers and quality control laboratories in the life sciences, pharmaceutical, and biotechnology industries for studying aspartyl proteases and related enzymes.

Application

• Protease Research: A key fluorogenic substrate for the study and characterization of aspartyl proteases, including cathepsin D and renin.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and evaluate potential protease inhibitors.
• Enzyme Kinetics: Enables the determination of kinetic parameters (Km, Vmax) for target proteases in vitro.
• Biochemical Assay Development: Serves as a core component in developing sensitive and specific activity assays for diagnostic or research purposes.
• Cell Biology Studies: Applied in cellular assays to monitor protease activity and localization within biological systems.
• Quality Control Testing: Used in biopharmaceutical manufacturing to assess the activity and consistency of enzyme-based products.

Basic Information

Product Name	H-Asp-Amc
CAS No.	219138-13-3
Molecular Formula	C₁₉H₂₃N₃O₇
Molecular Weight	405.40 g/mol
Synonyms	Aspartic Acid 7-Amido-4-methylcoumarin; Asp-AMC; L-Aspartic acid β-(7-amido-4-methylcoumarin); H-Aspartic acid-AMC; Aspartic acid AMC substrate; 7-Amido-4-methylcoumarin conjugate of L-aspartic acid; Fluorogenic substrate for aspartyl proteases; Cathepsin D substrate (AMC-based)
EINECS	Contact for details

Quality Control

Our H-Asp-Amc is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to relevant industry guidelines for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference in standard enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.