Description

Z-IETD-AFC is a fluorogenic peptide substrate specifically designed for the detection of caspase-8 activity. This compound is a critical reagent in life science research, enabling the study of apoptosis (programmed cell death) and related signaling pathways. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors who require reliable, high-purity biochemical tools for enzyme assays and drug discovery.

Application

• Caspase-8 Activity Assays: Primary substrate for fluorometric detection and quantification of caspase-8 enzyme activity in cell lysates and purified systems.
• Apoptosis Research: Used to study the extrinsic apoptosis pathway, helping to identify triggers and inhibitors of cell death.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to screen for novel compounds that modulate caspase-8 activity.
• Biochemical Characterization: Essential for determining enzyme kinetics (Km, Vmax) and specificity of caspase-8 and related proteases.
• Diagnostic Development: Used in the research and development of diagnostic kits for diseases involving dysregulated apoptosis, such as cancer and neurodegenerative disorders.
• Cell Biology Studies: Applied in flow cytometry and microscopy to detect caspase-8 activation in specific cell populations.

Basic Information

Product Name	Z-IETD-AFC
CAS No.	219138-02-0
Molecular Formula	C36H45FN6O12
Molecular Weight	772.78 g/mol
Synonyms	Ac-DEVD-AFC; Z-Ile-Glu-Thr-Asp-AFC; Caspase-8 Substrate (AFC); Ac-Ile-Glu-Thr-Asp-7-Amino-4-trifluoromethylcoumarin; Z-IETD-AFC, Fluorogenic Substrate; IETD-AFC; Ac-IETD-AFC; AFC Caspase-8 Substrate
EINECS	Contact for details

Quality Control

Our Z-IETD-AFC is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive biochemical assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant quality management standards to support research and development requiring reliable, publication-grade reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% of theoretical activity
Solubility	Soluble in DMSO, DMF
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.