Description

Z-Val-Ala-Asp-Amc CAS NO 219137-91-4 is a fluorogenic peptide substrate designed for the sensitive detection and quantification of caspase activity, specifically targeting caspase-3 and related enzymes. This compound is critical for life science research, enabling the study of apoptosis (programmed cell death) in various cellular and biochemical assays. It is an essential tool for researchers and pharmaceutical developers in oncology, neurobiology, and drug discovery who require reliable, high-purity biochemical reagents.

Application

• Caspase-3 Activity Assays: Primary fluorogenic substrate for in vitro enzymatic studies to measure caspase-3 activation and kinetics.
• Apoptosis Research: Used in cell-based assays to detect and quantify apoptosis induced by chemotherapeutic agents, toxins, or other stimuli.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize novel pro-apoptotic or anti-apoptotic compounds.
• Biochemical Pathway Analysis: A tool for investigating the caspase cascade and its role in diseases such as cancer, neurodegenerative disorders, and ischemia.
• Diagnostic Kit Development: Serves as a key component in the development of research-use-only (RUO) kits for apoptosis detection.
• Academic & Institutional Research: Fundamental reagent for universities and research institutes studying cell biology and death signaling pathways.

Basic Information

Product Name	Z-Val-Ala-Asp-Amc
CAS No.	219137-91-4
Molecular Formula	C30H37N5O9
Molecular Weight	611.65 g/mol
Synonyms	Z-VAD-AFC; Ac-DEVD-AFC; Caspase-3 Substrate (Ac-DEVD-AFC); Z-Val-Ala-Asp(OMe)-AFC; Z-VAD-AFC Caspase Substrate; Ac-Asp-Glu-Val-Asp-AFC; Ac-DEVD-AMC; N-Acetyl-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin
EINECS	Contact for details

Quality Control

Our Z-Val-Ala-Asp-Amc is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and functional activity testing in caspase-3 assays. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and assay performance is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF
Functional Activity	Conforms to reference standard in caspase-3 assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.