Description

Ac-Yvad-Afc CAS NO 219137-85-6 is a potent and selective fluorogenic substrate and irreversible inhibitor designed for the study of caspase-1 (interleukin-1β converting enzyme, ICE). This compound is a critical tool for researchers investigating apoptosis and inflammatory pathways. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic applications to measure caspase-1 activity with high sensitivity and specificity.

Application

• Biochemical Research: As a fluorogenic substrate for the continuous, real-time measurement of caspase-1 enzyme activity in cell lysates and purified enzyme preparations.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and evaluate potential caspase-1 inhibitors for drug discovery programs targeting inflammatory diseases.
• Apoptosis Studies: A key reagent for detecting and quantifying caspase-1 activation during programmed cell death and pyroptosis research.
• Diagnostic Development: Employed in the development of diagnostic kits and assays for monitoring inflammatory biomarkers and disease states.
• Enzyme Kinetics: Essential for determining kinetic parameters (Km, Vmax, kcat) and studying the mechanism of action of caspase-1.
• Cell Biology: Used in fluorescence microscopy and flow cytometry to visualize and measure caspase-1 activity in live or fixed cells.

Basic Information

Product Name	Ac-Yvad-Afc
CAS No.	219137-85-6
Molecular Formula	C27H36N4O9
Molecular Weight	560.60 g/mol
Synonyms	Ac-Tyr-Val-Ala-Asp-AFC; Ac-YVAD-AFC; Ac-YVAD-AFC Caspase-1 Substrate; ICE Substrate I, Fluorogenic; Caspase-1 Fluorogenic Substrate; Ac-YVAD-7-amino-4-trifluoromethylcoumarin; N-Acetyl-Tyr-Val-Ala-Asp-7-amino-4-trifluoromethylcoumarin; Interleukin-1β Converting Enzyme Substrate
EINECS	Contact for details

Quality Control

Our Ac-Yvad-Afc is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (by weight)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.