Description

H-Gly-Ala-Pro-Afc CAS NO 219137-81-2 is a specialized fluorogenic peptide substrate designed for the sensitive detection and measurement of protease enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise, high-throughput biochemical assays. It is primarily utilized in pharmaceutical research, diagnostic development, and biochemical studies focused on enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: Used as a selective substrate for measuring the activity of specific proteolytic enzymes in research and development.
• High-Throughput Screening (HTS): Enables rapid screening of potential drug candidates, particularly protease inhibitors, in pharmaceutical discovery.
• Diagnostic Kit Development: Serves as a key component in the formulation of diagnostic tests for diseases involving abnormal protease levels.
• Enzyme Kinetic Studies: Facilitates the detailed study of enzyme mechanisms, including determination of kinetic parameters like Km and Vmax.
• Biochemical Research: Employed in academic and industrial labs to investigate protease function in various biological pathways.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme-based products during production.

Basic Information

Product Name	H-Gly-Ala-Pro-Afc
CAS No.	219137-81-2
Molecular Formula	C₂₀H₂₅FN₄O₆
Molecular Weight	436.43 g/mol
Synonyms	Gly-Ala-Pro-AFC; H-Gly-Ala-Pro-7-Amino-4-trifluoromethylcoumarin; AFC Substrate GAP-AFC; 7-Amino-4-trifluoromethylcoumarin-Gly-Ala-Pro; Protease Substrate GAP-AFC; Fluorogenic Peptide Substrate GAP-AFC; Glycine-Alanine-Proline-AFC; GAP-AFC
EINECS	Contact for details

Quality Control

Our H-Gly-Ala-Pro-Afc is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.