Description

Fluorescein Diphosphate, Tetraammonium Salt is a high-purity, water-soluble enzymatic substrate critical for biochemical detection and analysis. This compound serves as a key reagent in phosphatase enzyme assays, where its enzymatic cleavage releases the highly fluorescent fluorescein molecule. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries requiring sensitive and quantitative enzymatic activity measurements. The product is supplied as a light-sensitive material to ensure optimal performance and stability.

Application

• Phosphatase Enzyme Assays: Primary substrate for detecting and quantifying alkaline phosphatase (ALP) and other phosphatase enzyme activities in research and diagnostic kits.
• Cell-Based Screening: Used in high-throughput screening (HTS) platforms to identify modulators of phosphatase signaling pathways in drug discovery.
• Immunoassay Development: Employed as a fluorescent reporter system in enzyme-linked immunosorbent assays (ELISA) and other immunoassays for enhanced sensitivity.
• Microbiology & Food Safety: Detection of bacterial contamination (e.g., E. coli) via microbial phosphatase activity in food and environmental testing.
• Biomedical Research: Tool for studying bone metabolism, liver function, and other physiological processes involving phosphatase enzymes.
• Quality Control (QC) Testing: Standard reagent for QC of diagnostic reagents and kits that utilize phosphatase enzymes.

Basic Information

Product Name	Fluorescein Diphosphate, Tetraammonium Salt
CAS No.	217305-49-2
Molecular Formula	C24H28N4O11P2 · xH2O
Molecular Weight	610.44 (Anhydrous)
Synonyms	Fluorescein Diphosphate Tetraammonium Salt; FDP Tetraammonium Salt; Fluorescein Diphosphate, Ammonium Salt; 3',6'-Bis(dihydrogen phosphate)fluorescein tetraammonium salt; ALP Substrate; Phosphatase Substrate FDP; Fluorescein diphosphate tetrammonium salt; FDPA
EINECS	Contact for details

Quality Control

Our Fluorescein Diphosphate, Tetraammonium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, desiccate at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Clear, yellow solution in water or buffer
Enzymatic Activity	Suitable for phosphatase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.