Description

Ac-Iepd-Amc is a specialized synthetic peptide derivative, identified by CAS number 216757-33-4, designed for advanced biochemical and pharmaceutical research. This compound serves as a critical substrate and building block in the study of protease activity and enzyme kinetics. It is essential for researchers and manufacturers in the fields of pharmaceutical development, proteomics, and diagnostic assay production, where precise molecular tools are required for high-throughput screening and mechanistic studies.

Application

• Protease Substrate: Used as a fluorogenic or chromogenic substrate for the detection and characterization of specific protease enzymes, including caspases and other proteolytic enzymes involved in cellular pathways.
• Biochemical Research: A key reagent in enzymatic assays to study inhibition kinetics, enzyme specificity, and catalytic mechanisms in academic and industrial R&D laboratories.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and validate new drug candidates that modulate protease activity for therapeutic applications.
• Diagnostic Development: Integral component in the development of in vitro diagnostic (IVD) kits for detecting disease biomarkers related to abnormal protease function.
• Cell Biology Studies: Utilized in apoptosis (programmed cell death) research to monitor caspase activation and other proteolytic events within live cells or cell lysates.

Basic Information

Product Name	Ac-Iepd-Amc
CAS No.	216757-33-4
Molecular Formula	C27H38N6O7
Molecular Weight	558.63 g/mol
Synonyms	Ac-Ile-Glu-Pro-Asp-AMC; Ac-IEPD-7-amido-4-methylcoumarin; Ac-Ile-Glu-Pro-Asp-AMC Fluorogenic Substrate; Caspase-8 Substrate I; Ac-IEPD-AFC (related); N-Acetyl-Ile-Glu-Pro-Asp-7-amido-4-methylcoumarin; ICE-L-III Protease Substrate; Ac-IEPD-AMC Caspase Substrate
EINECS	Contact for details

Quality Control

Our Ac-Iepd-Amc is manufactured under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption. For long-term stability, store desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard in enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.