Description

Bis(Sulfosuccinimidyl)Succinate Sodium Salt is a high-purity, water-soluble homobifunctional N-hydroxysulfosuccinimide (NHS) ester crosslinker. This compound is specifically engineered for the efficient conjugation of biomolecules, such as proteins and peptides, under mild aqueous conditions. It is an essential reagent for researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay production, where reliable and reproducible crosslinking is critical.

Application

• Bioconjugation and Protein Crosslinking: Primary reagent for covalently linking primary amine-containing molecules (e.g., antibodies, enzymes, peptides) to other amine-containing ligands or surfaces.
• Antibody-Drug Conjugate (ADC) Development: Used in the synthesis and research of ADCs to create stable linkages between monoclonal antibodies and cytotoxic payloads.
• Diagnostic Assay Manufacturing: Critical for immobilizing capture antibodies or antigens onto microplates, beads, or biosensor surfaces in ELISA, lateral flow, and other immunoassays.
• Biomaterial and Hydrogel Fabrication: Facilitates the crosslinking of amine-functionalized polymers to create stable, biocompatible networks for tissue engineering and drug delivery systems.
• Proteomics Research: Employed in chemical crosslinking mass spectrometry (XL-MS) to study protein-protein interactions and complex structures.
• Nucleic Acid Labeling: Can be used to conjugate amine-modified oligonucleotides to reporter molecules like fluorophores or enzymes.

Basic Information

Product Name	Bis(Sulfosuccinimidyl)Succinate Sodium Salt
CAS No.	215597-96-9
Molecular Formula	C16H12N2Na2O14S2
Molecular Weight	582.38 g/mol
Synonyms	BS3 Sodium Salt; BS3 Sodium Salt; Bis(sulfosuccinimidyl) Succinate Sodium Salt; Disuccinimidyl Sulfosuccinate Sodium Salt; BS3 (sulfo); 3,3'-Dithiobis(sulfosuccinimidyl propionate) (common mis-assignment); DTSSP (common mis-assignment); Bis(N-hydroxysulfosuccinimidyl) Succinate Sodium Salt; 1,4-Bis(sulfosuccinimidyl)butanedioate Disodium Salt
EINECS	Contact for details

Quality Control

Our Bis(Sulfosuccinimidyl)Succinate Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by UV)
Water Content (KF)	≤ 5.0%
Solubility	Freely soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.